Imperial College London
Alternate

DrDavidMann

Faculty of Natural Sciences, Department of Life Sciences

Reader in Cell Cycle Control
 
 
 
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Contact

 

+44 (0)20 7594 5302d.mann

 
 
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Location

 

605Sir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Vilar:2021:10.1038/s41467-020-20414-7,
author = {Vilar, Compte R and Summers, P and Lewis, B and Gonzalez-Garcia, J and Porreca, RM and Lim, A and Cadinu, P and Martin-Pintado, N and Mann, D and Edel, J and Vannier, JB and Kuimova, M and Vilar, Compte R},
doi = {10.1038/s41467-020-20414-7},
journal = {Nature Communications},
title = {Visualising G-quadruplex DNA dynamics in live cells by fluorescence lifetime imaging microscopy},
url = {http://dx.doi.org/10.1038/s41467-020-20414-7},
volume = {12},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Guanine rich regions of oligonucleotides fold into quadruple-stranded structures called G-quadruplexes (G4s). Increasing evidence suggests that these G4 structures form in vivo and play a crucial role in cellular processes. However, their direct observation in live cells remains a challenge. Here we demonstrate that a fluorescent probe (DAOTA-M2) in conjunction with fluorescence lifetime imaging microscopy (FLIM) can identify G4s within nuclei of live and fixed cells. We present a FLIM-based cellular assay to study the interaction of non-fluorescent small molecules with G4s and apply it to a wide range of drug candidates. We also demonstrate that DAOTA-M2 can be used to study G4 stability in live cells. Reduction of FancJ and RTEL1 expression in mammalian cells increases the DAOTA-M2 lifetime and therefore suggests an increased number of G4s in these cells, implying that FancJ and RTEL1 play a role in resolving G4 structures in cellulo.
AU  - Vilar,Compte R
AU  - Summers,P
AU  - Lewis,B
AU  - Gonzalez-Garcia,J
AU  - Porreca,RM
AU  - Lim,A
AU  - Cadinu,P
AU  - Martin-Pintado,N
AU  - Mann,D
AU  - Edel,J
AU  - Vannier,JB
AU  - Kuimova,M
AU  - Vilar,Compte R
DO  - 10.1038/s41467-020-20414-7
PY  - 2021///
SN  - 2041-1723
TI  - Visualising G-quadruplex DNA dynamics in live cells by fluorescence lifetime imaging microscopy
T2  - Nature Communications
UR  - http://dx.doi.org/10.1038/s41467-020-20414-7
UR  - http://hdl.handle.net/10044/1/85968
VL  - 12
ER  -
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