Imperial College London
Alternate

DrDavidMann

Faculty of Natural Sciences, Department of Life Sciences

Reader in Cell Cycle Control
 
 
 
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+44 (0)20 7594 5302d.mann

 
 
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Location

 

605Sir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Thinon:2014:10.1038/ncomms5919,
author = {Thinon, E and Serwa, RA and Broncel, M and Brannigan, JA and Brassat, U and Wright, MH and Heal, WP and Wilkinson, AJ and Mann, DJ and Tate, EW},
doi = {10.1038/ncomms5919},
journal = {Nature Communications},
pages = {1--13},
title = {Global profiling of co- and post-translationally N-myristoylated proteomes in human cells},
url = {http://dx.doi.org/10.1038/ncomms5919},
volume = {5},
year = {2014}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Protein N-myristoylation is a ubiquitous co- and post-translational modification that has been implicated in the development and progression of a range of human diseases. Here, we report the global N-myristoylated proteome in human cells determined using quantitative chemical proteomics combined with potent and specific human N-myristoyltransferase (NMT) inhibition. Global quantification of N-myristoylation during normal growth or apoptosis allowed the identification of >100 N-myristoylated proteins, >95% of which are identified for the first time at endogenous levels. Furthermore, quantitative dose response for inhibition of N-myristoylation is determined for >70 substrates simultaneously across the proteome. Small-molecule inhibition through a conserved substrate-binding pocket is also demonstrated by solving the crystal structures of inhibitor-bound NMT1 and NMT2. The presented data substantially expand the known repertoire of co- and post-translational N-myristoylation in addition to validating tools for the pharmacological inhibition of NMT in living cells.
AU  - Thinon,E
AU  - Serwa,RA
AU  - Broncel,M
AU  - Brannigan,JA
AU  - Brassat,U
AU  - Wright,MH
AU  - Heal,WP
AU  - Wilkinson,AJ
AU  - Mann,DJ
AU  - Tate,EW
DO  - 10.1038/ncomms5919
EP  - 13
PY  - 2014///
SN  - 2041-1723
SP  - 1
TI  - Global profiling of co- and post-translationally N-myristoylated proteomes in human cells
T2  - Nature Communications
UR  - http://dx.doi.org/10.1038/ncomms5919
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000342984100015&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://www.nature.com/articles/ncomms5919
UR  - http://hdl.handle.net/10044/1/18701
VL  - 5
ER  -
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