Imperial College London
Portrait Picture

Professor David W. McComb

Faculty of Engineering, Department of Materials

Adjunct Professor
 
 
 
//

Contact

 

+44 (0)20 7594 6750d.mccomb Website

 
 
//

Location

 

Royal School of MinesSouth Kensington Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Hou:2020:10.1038/s41565-019-0600-1,
author = {Hou, X and Zhang, X and Zhao, W and Zeng, C and Deng, B and McComb, DW and Du, S and Zhang, C and Li, W and Dong, Y},
doi = {10.1038/s41565-019-0600-1},
journal = {Nat Nanotechnol},
pages = {41--46},
title = {Vitamin lipid nanoparticles enable adoptive macrophage transfer for the treatment of multidrug-resistant bacterial sepsis.},
url = {http://dx.doi.org/10.1038/s41565-019-0600-1},
volume = {15},
year = {2020}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Sepsis, a condition caused by severe infections, affects more than 30 million people worldwide every year and remains the leading cause of death in hospitals1,2. Moreover, antimicrobial resistance has become an additional challenge in the treatment of sepsis3, and thus, alternative therapeutic approaches are urgently needed2,3. Here, we show that adoptive transfer of macrophages containing antimicrobial peptides linked to cathepsin B in the lysosomes (MACs) can be applied for the treatment of multidrug-resistant bacteria-induced sepsis in mice with immunosuppression. The MACs are constructed by transfection of vitamin C lipid nanoparticles that deliver antimicrobial peptide and cathepsin B (AMP-CatB) mRNA. The vitamin C lipid nanoparticles allow the specific accumulation of AMP-CatB in macrophage lysosomes, which is the key location for bactericidal activities. Our results demonstrate that adoptive MAC transfer leads to the elimination of multidrug-resistant bacteria, including Staphylococcus aureus and Escherichia coli, leading to the complete recovery of immunocompromised septic mice. Our work provides an alternative strategy for overcoming multidrug-resistant bacteria-induced sepsis and opens up possibilities for the development of nanoparticle-enabled cell therapy for infectious diseases.
AU  - Hou,X
AU  - Zhang,X
AU  - Zhao,W
AU  - Zeng,C
AU  - Deng,B
AU  - McComb,DW
AU  - Du,S
AU  - Zhang,C
AU  - Li,W
AU  - Dong,Y
DO  - 10.1038/s41565-019-0600-1
EP  - 46
PY  - 2020///
SP  - 41
TI  - Vitamin lipid nanoparticles enable adoptive macrophage transfer for the treatment of multidrug-resistant bacterial sepsis.
T2  - Nat Nanotechnol
UR  - http://dx.doi.org/10.1038/s41565-019-0600-1
UR  - https://www.ncbi.nlm.nih.gov/pubmed/31907443
VL  - 15
ER  -
Download