Imperial College London
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Professor David W. McComb

Faculty of Engineering, Department of Materials

Adjunct Professor
 
 
 
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Contact

 

+44 (0)20 7594 6750d.mccomb Website

 
 
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Location

 

Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Liu:2021:10.3791/62581,
author = {Liu, Y and Lee, Y-U and Yi, T and Wu, K and Bouchet-Marquis, C and Chan, H and Breuer, CK and McComb, DW},
doi = {10.3791/62581},
journal = {J Vis Exp},
title = {Surgery and Sample Processing for Correlative Imaging of the Murine Pulmonary Valve.},
url = {http://dx.doi.org/10.3791/62581},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The underlying causes of heart valve related-disease (HVD) are elusive. Murine animal models provide an excellent tool for studying HVD, however, the surgical and instrumental expertise required to accurately quantify the structure and organization across multiple length scales have stunted its advancement. This work provides a detailed description of the murine dissection, en bloc staining, sample processing, and correlative imaging procedures for depicting the heart valve at different length scales. Hydrostatic transvalvular pressure was used to control the temporal heterogeneity by chemically fixing the heart valve conformation. Micro-computed tomography (µCT) was used to confirm the geometry of the heart valve and provide a reference for the downstream sample processing needed for the serial block face scanning electron microscopy (SBF-SEM). High-resolution serial SEM images of the extracellular matrix (ECM) were taken and reconstructed to provide a local 3D representation of its organization. µCT and SBF-SEM imaging methods were then correlated to overcome the spatial variation across the pulmonary valve. Though the work presented is exclusively on the pulmonary valve, this methodology could be adopted for describing the hierarchical organization in biological systems and is pivotal for the structural characterization across multiple length scales.
AU  - Liu,Y
AU  - Lee,Y-U
AU  - Yi,T
AU  - Wu,K
AU  - Bouchet-Marquis,C
AU  - Chan,H
AU  - Breuer,CK
AU  - McComb,DW
DO  - 10.3791/62581
PY  - 2021///
TI  - Surgery and Sample Processing for Correlative Imaging of the Murine Pulmonary Valve.
T2  - J Vis Exp
UR  - http://dx.doi.org/10.3791/62581
UR  - https://www.ncbi.nlm.nih.gov/pubmed/34424247
ER  -
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