Publications
132 results found
Morris DJ, Adler ID, Robinson TJ, 1993, Somatic cell hybrid mapping on mouse chromosome 11 (MMU11): assignment of markers relative to two breakpoints in band D., Genomics, Vol: 15, Pages: 323-331, ISSN: 0888-7543
Mouse x rat somatic cell hybrids were generated by fusing mouse cell lines that are heterozygous for reciprocal translocations involving the T42H and T9Ad breakpoints on mouse chromosome 11 (MMU11) to a thymidine kinase-negative (Tk-) rat cell line, RT2Tk-. Selection in HAT medium with geneticin disulfate (G418) resulted in some hybrid clones retaining only one derivative translocation chromosome with that part of MMU11 carrying the Tk-1 locus. Southern blot and PCR analyses of these hybrids were used to map the two breakpoints and 30 markers relative to them. The T42H breakpoint has been localized between Mpo and the Cola-1/Hox-2 cluster of loci and is proximal to the T9Ad breakpoint. The T9Ad breakpoint is proximal to the distal loci Tk-1, Gaa, D11Jkn1, and P4hb. The positions of 14 loci (Hox-2, Cola-1, Rara, Phb, Erba, Rnula-1, D11Pas1, Gfap, D11Mit13, D11Mit11, D11Mit12, Myla, Empb3 and Gh) have been further refined by their localization between the two breakpoints in band D. This study therefore improves the correlation of the genetic and physical maps of MMU11 and extends the known homology between MMU11 and human chromosome 17 (HSA17) by the assignment of three additional HSA17 markers, the profilin gene, Pfn, an anonymous marker, D17s28h, and the Crk oncogene, to above the T42H breakpoint; and the prohibitin gene, Phb, to between the T42H and T9Ad breakpoints in band D on MMU11.
Robinson TJ, Morris DJ, Fairall N, 1991, Interspecific hybridization in the bovidae: Sterility of Alcelaphus buselaphus × Damaliscus dorcas F1 progeny, Biological Conservation, Vol: 58, Pages: 345-356, ISSN: 0006-3207
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Morris DJ, Heim RA, Verga V, et al., 1991, Study of 30 DNA markers in three southern African populations., Gene Geogr, Vol: 5, Pages: 1-12, ISSN: 0394-249X
Thirty anonymous DNA markers were investigated in Southern African Caucasoid, Negroid and San populations. Sixteen of these are new markers that were developed in our laboratory; the remainder are closely linked to the cystic fibrosis locus on chromosome 7. Average heterozygosity in the Caucasoid and Negroid populations was calculated at the loci identified by each of the anonymous probes, using two approaches, and was found to be .0020 and .0030 for the Caucasoid population and .0023 and .0025 for the Negroid population. Variation between populations (measured by FST) and between markers was calculated from allele frequency data gathered for all markers in the three populations. Significant differences in allele frequency between the populations were observed for the cystic fibrosis markers MET D, MET H and 7C22, with little or no variation observed in the Negroid and San populations. Mean heterozygosity (D) was found to be considerably lower in San (.250) than in Caucasoid (.373) and Negroid populations (.0320) and possible explanations for this are provided. The smallest genetic distance (60 x 10(-3)) was found between the Negroid and San populations, and the greatest distance between the Caucasoid and San populations (167 x 10(-3)).
Morris DJ, Robinson TJ, 1991, Thymidine kinase (Tk-1) maps below the T42H breakpoint on mouse chromosome 11., Mamm Genome, Vol: 1, Pages: 263-264, ISSN: 0938-8990
Robinson TJ, Morris DJ, Ledbetter DH, 1990, Chromosomal assignment and regional localization of myeloperoxidase in the mouse., Cytogenet Cell Genet, Vol: 53, Pages: 83-86, ISSN: 0301-0171
Using a rat x mouse somatic cell hybrid that contains chromosome 11 as the only mouse material, we have shown that myeloperoxidase, which maps to human chromosome 17, maps to mouse chromosome 11. Regional assignment of the gene by in situ hybridization localized Mpo to the region C-E1, with a peak at band 11C. These results further confirm and extend observations on the remarkable homology between human chromosome 17 and mouse 11.
Morris DJ, dos Santos M, Povey S, et al., 1989, A new marker on chromosome 15, D15S74, detects three RFLPs., Nucleic Acids Res, Vol: 17, ISSN: 0305-1048
Morris DJ, dos Santos M, Povey S, et al., 1989, A new anonymous marker on chromosome 11, D11S347, detects two TaqI RFLPs., Nucleic Acids Res, Vol: 17, ISSN: 0305-1048
Morris DJ, dos Santos M, Ramsay M, et al., 1989, An anonymous single copy probe, D3S153, detects an SstI RFLP., Nucleic Acids Res, Vol: 17, ISSN: 0305-1048
Morris DJ, dos Santos M, Povey S, et al., 1989, A new marker on chromosome 4q, D4S138, detects two RFLPs., Nucleic Acids Res, Vol: 17, ISSN: 0305-1048
Morris DJ, dos Santos M, Povey S, et al., 1989, A new anonymous marker on chromosome 7, D7S420, identifies a PvuII RFLP., Nucleic Acids Res, Vol: 17, ISSN: 0305-1048
Rosendorff J, Jacobson MJ, Morris D, et al., 1989, First trimester prenatal diagnosis by chorionic villus sampling. The Johannesburg experience with 48 cases., S Afr Med J, Vol: 75, Pages: 15-17, ISSN: 0256-9574
Chorionic villus sampling (CVS) is a first trimester alternative to amniocentesis for the prenatal detection of genetic disorders. Initial experience in 48 patients, in whom transcervical CVS was utilised for the diagnosis of chromosomal, biochemical or molecular disorders, is reported. An adequate villus sample was obtained in all cases and a diagnostic result was achieved in 90% of cases. In this series, the miscarriage rate was 4.2%. It is concluded that CVS appears to be a relatively safe and reliable procedure, but the risk of miscarriage can only be accurately assessed after further investigation.
Morris DJ, van Aarde RJ, 1985, Sexual behavior of the female porcupine Hystrix africaeaustralis., Horm Behav, Vol: 19, Pages: 400-412, ISSN: 0018-506X
Porcupines are sexually active throughout the estrous cycle, and sexual behavior is not affected by the reproductive status and hormonal milieu of females. Increased female-male interactions during estrus are the only indications of behavioral changes during estrus. The lack of aggression shown by females to known males as opposed to aggression shown to strange males, the greater interest shown by males toward certain females, and the superior breeding success of these females suggest a pair-bonding mating system. Limited changes in female receptivity throughout the cycle might be of importance in maintaining the pair-bond.
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