Imperial College London
Portrait Picture

ProfessorDarrylOverby

Faculty of Engineering, Department of Bioengineering

Professor of Mechanobiology
 
 
 
//

Contact

 

+44 (0)20 7594 6376d.overby

 
 
//

Location

 

3.07Bessemer BuildingSouth Kensington Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Madekurozwa:2022:10.1016/j.exer.2022.109103,
author = {Madekurozwa, M and Reina-Torres, E and Overby, DR and van, Batenburg-Sherwood J},
doi = {10.1016/j.exer.2022.109103},
journal = {Experimental Eye Research},
pages = {109103--109103},
title = {Measurement of postmortem outflow facility using iPerfusion.},
url = {http://dx.doi.org/10.1016/j.exer.2022.109103},
volume = {220},
year = {2022}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The key risk factor for glaucoma is elevation of intraocular pressure (IOP) and alleviating it is the only effective therapeutic approach to inhibit further vision loss. IOP is regulated by the flow of aqueous humour across resistive tissues, and a reduction in outflow facility, is responsible for the IOP elevation in glaucoma. Measurement of outflow facility is therefore important when investigating the pathophysiology of glaucoma and testing candidate treatments for lowering IOP. Due to similar anatomy and response to pharmacological treatments, mouse eyes are a common model of human aqueous humour dynamics. The ex vivo preparation, in which an enucleated mouse eye is mounted in a temperature controlled bath and cannulated, has been well characterised and is widely used. The postmortem in situ model, in which the eyes are perfused within the cadaver, has received relatively little attention. In this study, we investigate the postmortem in situ model using the iPerfusion system, with a particular focus on i) the presence or absence of pressure-independent flow, ii) the effect of evaporation on measured flow rates and iii) the magnitude and pressure dependence of outflow facility and how these properties are affected by postmortem changes. Measurements immediately after cannulation and following multi-pressure facility measurement demonstrated negligible pressure-independent flow in postmortem eyes, in contrast to assumptions made in previous studies. Using a humidity chamber, we investigated whether the humidity of the surrounding air would influence measured flow rates. We found that at room levels of humidity, evaporation of saline droplets on the eye resulted in artefactual flow rates with a magnitude comparable to outflow, which were eliminated by a high relative humidity (>85%) environment. Average postmortem outflow facility was ∼4 nl/min/mmHg, similar to values observed ex vivo, irrespective of whether a postmortem delay was introduced prior to c
AU  - Madekurozwa,M
AU  - Reina-Torres,E
AU  - Overby,DR
AU  - van,Batenburg-Sherwood J
DO  - 10.1016/j.exer.2022.109103
EP  - 109103
PY  - 2022///
SN  - 0014-4835
SP  - 109103
TI  - Measurement of postmortem outflow facility using iPerfusion.
T2  - Experimental Eye Research
UR  - http://dx.doi.org/10.1016/j.exer.2022.109103
UR  - https://www.ncbi.nlm.nih.gov/pubmed/35525299
UR  - https://www.sciencedirect.com/science/article/pii/S001448352200183X?via%3Dihub
UR  - http://hdl.handle.net/10044/1/97306
VL  - 220
ER  -
Download