Imperial College London
Alternate

Professor David J. Payne

Faculty of Engineering, Department of Materials

Professor of Materials Chemistry
 
 
 
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Contact

 

+44 (0)20 7594 2585d.payne Website

 
 
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Location

 

2.09Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Trantidou:2018:10.1016/j.snb.2018.03.165,
author = {Trantidou, T and Regoutz, A and Voon, X and Payne, D and Ces, O},
doi = {10.1016/j.snb.2018.03.165},
journal = {Sensors and Actuators B: Chemical},
pages = {34--41},
title = {A “cleanroom-free” and scalable manufacturing technology for the microfluidic generation of lipid-stabilized droplets and cell-sized multisomes},
url = {http://dx.doi.org/10.1016/j.snb.2018.03.165},
volume = {267},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - There is a growing demand to construct artificial biomimetic structures from the bottom-up using simple chemical components in a controlled and high-throughput way. These cell mimics are encapsulated by lipid membranes and can reconstitute biological machinery within them. To date, such synthetic cells based upon droplet microfluidics are fabricated using non-scalable, expensive and time-consuming strategies, and are thus restricted to small-scale in-house manufacturing. Here, we report a “cleanroom-free” and highly scalable microfluidic manufacturing technology based on dry film resists and multilayer lamination. The technology facilitates the controlled and high-throughput generation of stable and monodisperse droplets using anionic surfactants and more biologically relevant phospholipids. We demonstrate the versatility of this approach by selectively patterning the surface chemistry of the device, enabling the production of compartmentalized lipid structures based on droplet interface bilayers (multisomes). This technology has the potential to simultaneously unlock the widespread exploitation of microfluidics to chemists and synthetic biologists not having access to controlled production environments and facilitate low-cost (<£1) high-volume fabrication of self-contained disposable devices with minimum feature sizes of 30μm. The associated material and equipment costs approach those of other deskilled prototyping technologies, such as 3D printing that have made the transition into the mainstream.
AU  - Trantidou,T
AU  - Regoutz,A
AU  - Voon,X
AU  - Payne,D
AU  - Ces,O
DO  - 10.1016/j.snb.2018.03.165
EP  - 41
PY  - 2018///
SN  - 0925-4005
SP  - 34
TI  - A “cleanroom-free” and scalable manufacturing technology for the microfluidic generation of lipid-stabilized droplets and cell-sized multisomes
T2  - Sensors and Actuators B: Chemical
UR  - http://dx.doi.org/10.1016/j.snb.2018.03.165
UR  - http://hdl.handle.net/10044/1/58625
VL  - 267
ER  -
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