Imperial College London
Dr David Pitcher

Dr David Pitcher

Central Faculty, Enterprise

Imperial College Advanced Hackspace Fellow
 
 
 
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Contact

 

d.pitcher

 
 
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Location

 

123Stadium HouseWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Houston:2018:10.1016/j.yjmcc.2018.05.002,
author = {Houston, CPJ and Tzortzis, KN and Roney, C and Saglietto, A and Pitcher, DS and Cantwell, C and Chowdhury, RA and Ng, FS and Peters, NS and Dupont, E},
doi = {10.1016/j.yjmcc.2018.05.002},
journal = {Journal of Molecular and Cellular Cardiology},
pages = {155--164},
title = {Characterisation of re-entrant circuit (or rotational activity) in vitro using the HL1-6 myocyte cell line},
url = {http://dx.doi.org/10.1016/j.yjmcc.2018.05.002},
volume = {119},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Fibrillation is the most common arrhythmia observed in clinical practice. Understanding of the mechanisms underlying its initiation and maintenance remains incomplete. Functional re-entries are potential drivers of the arrhythmia. Two main concepts are still debated, the “leading circle” and the “spiral wave or rotor” theories. The homogeneous subclone of the HL1 atrial-derived cardiomyocyte cell line, HL1-6, spontaneously exhibits re-entry on a microscopic scale due to its slow conduction velocity and the presence of triggers, making it possible to examine re-entry at the cellular level.We therefore investigated the re-entry cores in cell monolayers through the use of fluorescence optical mapping at high spatiotemporal resolution in order to obtain insights into the mechanisms of re-entry.Re-entries in HL1-6 myocytes required at least two triggers and a minimum colony area to initiate (3.5 to 6.4mm2). After electrical activity was completely stopped and re-started by varying the extracellular K+ concentration, re-entries never returned to the same location while 35% of triggers re-appeared at the same position. A conduction delay algorithm also allows visualisation of the core of the re-entries. This work has revealed that the core of re-entries is conduction blocks constituted by lines and/or groups of cells rather than the round area assumed by the other concepts of functional re-entry. This highlights the importance of experimentation at the microscopic level in the study of re-entry mechanisms.
AU  - Houston,CPJ
AU  - Tzortzis,KN
AU  - Roney,C
AU  - Saglietto,A
AU  - Pitcher,DS
AU  - Cantwell,C
AU  - Chowdhury,RA
AU  - Ng,FS
AU  - Peters,NS
AU  - Dupont,E
DO  - 10.1016/j.yjmcc.2018.05.002
EP  - 164
PY  - 2018///
SN  - 0022-2828
SP  - 155
TI  - Characterisation of re-entrant circuit (or rotational activity) in vitro using the HL1-6 myocyte cell line
T2  - Journal of Molecular and Cellular Cardiology
UR  - http://dx.doi.org/10.1016/j.yjmcc.2018.05.002
UR  - http://hdl.handle.net/10044/1/59778
VL  - 119
ER  -
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