Publications
250 results found
Zhang D, Trzcinski AP, Kunacheva C, et al., 2016, Characterization of soluble microbial products (SMPs) in a membrane bioreactor (MBR) treating synthetic wastewater containing pharmaceutical compounds, Water Research, Vol: 102, Pages: 594-606, ISSN: 0043-1354
This study investigated the behaviour and characteristics of soluble microbial products (SMP) in two anoxic-aerobic membrane bioreactors (MBRs): MBRcontrol and MBRpharma, for treating municipal wastewater. Both protein and polysaccharides measured exhibited higher concentrations in the MBRpharma than the MBRcontrol. Molecular weight (MW) distribution analysis revealed that the presence of pharmaceuticals enhanced the accumulation of SMPs with macro- (13,091 kDa and 1587 kDa) and intermediate-MW (189 kDa) compounds in the anoxic MBRpharma, while a substantial decrease was observed in both MBR effluents. Excitation emission matrix (EEM) fluorescence contours indicated that the exposure to pharmaceuticals seemed to stimulate the production of aromatic proteins containing tyrosine (10.1–32.6%) and tryptophan (14.7–43.1%), compared to MBRcontrol (9.9–29.1% for tyrosine; 11.8–42.5% for tryptophan). Gas chromatography–mass spectrometry (GC-MS) analysis revealed aromatics, long-chain alkanes and esters were the predominant SMPs in the MBRs. More peaks were present in the aerobic MBRpharma (196) than anoxic MBRpharma (133). The SMPs identified exhibited both biodegradability and recalcitrance in the MBR treatment processes. Only 8 compounds in the MBRpharma were the same as in the MBRcontrol. Alkanes were the most dominant SMPs (51%) in the MBRcontrol, while aromatics were dominant (40%) in the MBRpharma. A significant decrease in aromatics (from 16 to 7) in the MBRpharma permeate was observed, compared to the aerobic MBRpharma. Approximately 21% of compounds in the aerobic MBRcontrol were rejected by membrane filtration, while this increased to 28% in the MBRpharma.
Ward K, Cheng SI, Stuckey DC, 2016, Protein separation using non-ionic and cationic surfactant precipitation, Journal of Chemical Technology and Biotechnology, ISSN: 1097-4660
BACKGROUND: The predominant use of anionic surfactants to precipitate high isoelectric point (pI) proteins has increased in recent years, simplifying downstream separations. However, few researchers have tested cationic and non-ionic surfactants, whose properties are more desirable. This paper examines the effect of these surfactants on the precipitation efficiency of lysozyme, trypsin inhibitor and bovine serum albumin (BSA). RESULTS: Precipitation of BSA and trypsin inhibitor using the cationics, trioctylmethylammonium chloride (TOMAC) and dimethyl dioctadecyl ammonium chloride (DODMAC) was evaluated, with TOMAC being superior. More than 90% of BSA was precipitated using TOMAC at pH 9.0 with a molar ratio of surfactant/protein (R) of 100:1, while 88% was precipitated using DODMAC. However, for trypsin inhibitor, only 58% was precipitated at an R of 61:1 and pH 6.2 using TOMAC. Protein precipitate recovery using the anionic surfactant sodium bis-[2-ethylhexyl] sulfosuccinate (AOT) was effective only with trypsin inhibitor, with 100% of the protein being recovered. CONCLUSIONS: This study shows the potential of cationics to precipitate low pI proteins, and recover them using the counterionic surfactant AOT, with 100% recovery of trypsin inhibitor. However, non-ionic surfactants were ineffective. The method not only separates, but also preserves protein structure; hence cationic surfactants for low pI protein separation are promising.
Ketheesan B, Pham MT, Stuckey DC, 2016, Iron deficiency and bioavailability in anaerobic batch and submerged membrane bioreactors (SAMBR) during organic shock loads, Bioresource Technology, Vol: 211, Pages: 136-145, ISSN: 1873-2976
Le C, Kunacheva C, Stuckey DC, 2016, "Protein" Measurement in Biological Wastewater Treatment Systems: A Critical Evaluation, ENVIRONMENTAL SCIENCE & TECHNOLOGY, Vol: 50, Pages: 3074-3081, ISSN: 0013-936X
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- Citations: 72
Lin YZ, Guo M, Shah N, et al., 2016, Economic and environmental evaluation of nitrogen removal and recovery methods from wastewater, Bioresource Technology, Vol: 215, Pages: 227-238, ISSN: 1873-2976
The driver for waste-based economic growth is long-term strategic design, and a paradigm-shift from waste treatment to resource recovery. This study aims to use an integrated modelling approach to evaluate the holistic economic and environmental profiles of three alternative nitrogen removal and recovery methods integrated into wastewater treatment systems, including conventional nitrification-denitrification, Anammox, and the anaerobic ion exchange route, to provide insights into N recovery system designs which are key elements in building a sustainable circular economy. Our results suggest that ion exchange is a promising technology showing high N removal-recovery efficiency from municipal wastewater and delivering competitive sustainability scores. In comparison with the well-developed conventional route, ion exchange and Anammox are undergoing significant research and development; as highlighted in sensitivity analyses, there is considerable room for process design and optimization of ion exchange systems to achieve economically and environmentally optimal performance.
Le C, Stuckey DC, 2016, Colorimetric measurement of carbohydrates in biological wastewater treatment systems: A critical evaluation, Water Research, Vol: 94, Pages: 280-287, ISSN: 1879-2448
Trzcinski AP, Stuckey DC, 2016, Effect of sparging rate on permeate quality in a submerged anaerobic membrane bioreactor (SAMBR) treating leachate from the organic fraction of municipal solid waste (OFMSW), JOURNAL OF ENVIRONMENTAL MANAGEMENT, Vol: 168, Pages: 67-73, ISSN: 0301-4797
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- Citations: 16
Trzcinski AP, Stuckey DC, 2016, Inorganic fouling of an anaerobic membrane bioreactor treating leachate from the organic fraction of municipal solid waste (OFMSW) and a polishing aerobic membrane bioreactor, BIORESOURCE TECHNOLOGY, Vol: 204, Pages: 17-25, ISSN: 0960-8524
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- Citations: 32
Ward K, Stuckey DC, 2016, Refractive index matching to develop transparent polyaphrons: Characterization of immobilized proteins, Colloids and Surfaces B - Biointerfaces, Vol: 142, Pages: 159-164, ISSN: 1873-4367
Refractive index matching was used to create optically transparent polyaphrons to enable proteins adsorbed to the aphron surface to be characterized. Due to the significant light scattering created by polyaphrons, refractive index matching allowed for representative circular dichroism (CD) spectra and acceptable structural characterization. The method utilized n-hexane as the solvent phase, a mixture of glycerol and phosphate buffer (30% [w/v]) as the aqueous phase, and the non-ionic surfactants, Laureth-4 and Kolliphor P-188. Deconvolution of CD spectra revealed that the immobilized protein adapted its native conformation, showing that the adsorbed protein interacted only with the bound water layer (“soapy shell”) of the aphron. Isothermal calorimetry further demonstrated that non-ionic surfactant interactions were virtually non-existent, even at the high concentrations used (5% [w/v]), proving that non-ionic surfactants can preserve protein conformation.
Cheng SI, Stuckey DC, 2016, Protein separation mechanisms in surfactant precipitation systems, SEPARATION SCIENCE AND TECHNOLOGY, Vol: 51, Pages: 181-191, ISSN: 0149-6395
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- Citations: 4
Pham MT, Ketheesan B, Yan Z, et al., 2015, Trace metal speciation and bioavailability in anaerobic digestion: A review, BIOTECHNOLOGY ADVANCES, Vol: 34, Pages: 122-136, ISSN: 0734-9750
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- Citations: 189
Ketheesan B, Stuckey DC, 2015, Effects of Hydraulic/Organic Shock/Transient Loads in Anaerobic Wastewater Treatment: A Review, CRITICAL REVIEWS IN ENVIRONMENTAL SCIENCE AND TECHNOLOGY, Vol: 45, Pages: 2693-2727, ISSN: 1064-3389
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- Citations: 56
Ward K, Xi J, Stuckey DC, 2015, Immobilization of enzymes using non-ionic colloidal liquid aphrons (CLAs): Surface and enzyme effects, COLLOIDS AND SURFACES B-BIOINTERFACES, Vol: 136, Pages: 424-430, ISSN: 0927-7765
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- Citations: 3
Liu Y, Yu H-Q, Ng WJ, et al., 2015, Wastewater-Energy Nexus Preface, CHEMOSPHERE, Vol: 140, Pages: 1-1, ISSN: 0045-6535
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- Citations: 6
Puchongkawarin C, Gomez-Mont C, Stuckey DC, et al., 2015, Optimization-based methodology for the development of wastewater facilities for energy and nutrient recovery, CHEMOSPHERE, Vol: 140, Pages: 150-158, ISSN: 0045-6535
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- Citations: 53
Herrero M, Stuckey DC, 2015, Bioaugmentation and its application in wastewater treatment: A review, CHEMOSPHERE, Vol: 140, Pages: 119-128, ISSN: 0045-6535
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- Citations: 288
Chen JL, Steele TWJ, Stuckey DC, 2015, Modeling and Application of a Rapid Fluorescence-Based Assay for Biotoxicity in Anaerobic Digestion, ENVIRONMENTAL SCIENCE & TECHNOLOGY, Vol: 49, Pages: 13463-13471, ISSN: 0013-936X
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- Citations: 34
Ward K, Xi J, Stuckey DC, 2015, mmobilization of enzymes using non-ionic colloidal liquid aphrons (CLAs): Activity kinetics, conformation, and energetics, Biotechnology and Bioengineering, Vol: 113, Pages: 970-978, ISSN: 1097-0290
Trzcinski AP, Stuckey DC, 2015, Contribution of acetic acid to the hydrolysis of lignocellulosic biomass under abiotic conditions, BIORESOURCE TECHNOLOGY, Vol: 185, Pages: 441-444, ISSN: 0960-8524
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- Citations: 18
Chen JL, Ortiz R, Xiao Y, et al., 2015, Rapid fluorescence-based measurement of toxicity in anaerobic digestion, WATER RESEARCH, Vol: 75, Pages: 123-130, ISSN: 0043-1354
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- Citations: 17
Xiao Y, De Araujo C, Sze CC, et al., 2015, Toxicity measurement in biological wastewater treatment processes: A review, JOURNAL OF HAZARDOUS MATERIALS, Vol: 286, Pages: 15-29, ISSN: 0304-3894
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- Citations: 85
Xiao Y, De Araujo C, Sze CC, et al., 2015, Controlling a toxic shock of pentachlorophenol (PCP) to anaerobic digestion using activated carbon addition, BIORESOURCE TECHNOLOGY, Vol: 181, Pages: 303-311, ISSN: 0960-8524
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- Citations: 14
Fox RA, Stuckey DC, 2015, The effect of sparging rate on transmembrane pressure and critical flux in an AnMBR, JOURNAL OF ENVIRONMENTAL MANAGEMENT, Vol: 151, Pages: 280-285, ISSN: 0301-4797
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- Citations: 35
Teli A, Vyrides I, Stuckey DC, 2015, Treatment of metalworking fluids using a submerged anaerobic membrane bioreactor (SAMBR), JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, Vol: 90, Pages: 507-513, ISSN: 0268-2575
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- Citations: 12
Fox R, Stuckey DC, 2015, MS-2 and T4 phage removal in an anaerobic membrane bioreactor (AnMBR): effect of gas sparging rate, JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, Vol: 90, Pages: 384-390, ISSN: 0268-2575
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- Citations: 15
Zhang D, Hua T, Xiao F, et al., 2015, Phytotoxicity and bioaccumulation of ZnO nanoparticles in <i>Schoenoplectus tabernaemontani</i>, CHEMOSPHERE, Vol: 120, Pages: 211-219, ISSN: 0045-6535
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- Citations: 55
Chen JL, Ortiz R, Steele TWJ, et al., 2014, Toxicants inhibiting anaerobic digestion: A review, BIOTECHNOLOGY ADVANCES, Vol: 32, Pages: 1523-1534, ISSN: 0734-9750
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- Citations: 394
Vyrides I, Bonakdarpour B, Stuckey DC, 2014, Salinity effects on biodegradation of Reactive Black 5 for one stage and two stages sequential anaerobic aerobic biological processes employing different anaerobic sludge, INTERNATIONAL BIODETERIORATION & BIODEGRADATION, Vol: 95, Pages: 294-300, ISSN: 0964-8305
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- Citations: 17
Kunacheva C, Stuckey DC, 2014, Analytical methods for soluble microbial products (SMP) and extracellular polymers (ECP) in wastewater treatment systems: A review, WATER RESEARCH, Vol: 61, Pages: 1-18, ISSN: 0043-1354
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- Citations: 181
Johari K, Sarnan N, Song ST, et al., 2013, Utilization of Coconut Milk Processing Waste as a Low-Cost Mercury Sorbent, INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH, Vol: 52, Pages: 15648-15657, ISSN: 0888-5885
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- Citations: 33
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