Imperial College London
Dr Dagmar Tapon

DrDagmarTapon

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Honorary Senior Lecturer
 
 
 
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Contact

 

+44 (0)20 3313 3587d.tapon

 
 
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Location

 

2020Queen Charlottes and Chelsea HospitalHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Lawin:2016:10.1002/pd.4843,
author = {Lawin, O'Brien A and Dall'Asta, A and Tapon, D and Mann, K and Ahn, JW and Ellis, R and Ogilvie, C and Lees, C},
doi = {10.1002/pd.4843},
journal = {Prenatal Diagnosis},
pages = {708--713},
title = {Gestation related karyotype, QF-PCR and CGH-array failure rates in diagnostic amniocentesis},
url = {http://dx.doi.org/10.1002/pd.4843},
volume = {36},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - BACKGROUND: Few data exist describing laboratory related failure rates in prenatal diagnosis. The aim of this study is to assess the laboratory associated failure rate for karyotype, QF-PCR and CGH-array following amniocentesis in relation to gestation. METHODS: Retrospective database study of amniocenteses performed 2004-2014 comparing laboratory failure rate for karyotype, QF-PCR and CGH-array between 16 + 0 and 40 + 0 weeks' gestation. RESULTS: A total of 10 484 amniotic fluid test results were collected in three databases. Karyotype failed in 41/1797 (2.3%) tests; failure rate was significantly greater with advancing gestation reaching 43% at 36-40 weeks. QF-PCR failed in 132/5715 tests (2.3%) and was significantly greater with advancing gestation reaching 7% at 36-40 weeks. For CGH-array, 10/298 tests (3.4%) failed analysis. In one case, no result was obtainable by any technique. CONCLUSIONS: These data provide gestation specific laboratory failure rates for amniocentesis enabling informed decisions about the timing and laboratory technique most applicable to the clinical situation. Before 20 weeks, karyotype is least likely to fail of the three techniques. However, in the late third trimester, QF-PCR and, in particular, karyotyping are more likely to fail than CGH-array. Although there is some overlap between the three different tests, they may be preferentially offered in different clinical scenarios. © 2016 John Wiley & Sons, Ltd.
AU  - Lawin,O'Brien A
AU  - Dall'Asta,A
AU  - Tapon,D
AU  - Mann,K
AU  - Ahn,JW
AU  - Ellis,R
AU  - Ogilvie,C
AU  - Lees,C
DO  - 10.1002/pd.4843
EP  - 713
PY  - 2016///
SN  - 1097-0223
SP  - 708
TI  - Gestation related karyotype, QF-PCR and CGH-array failure rates in diagnostic amniocentesis
T2  - Prenatal Diagnosis
UR  - http://dx.doi.org/10.1002/pd.4843
UR  - http://hdl.handle.net/10044/1/43730
VL  - 36
ER  -
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