Imperial College London
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DrDesWalsh

Faculty of Medicine, Faculty of Medicine Centre

Director of Research Strategy
 
 
 
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Contact

 

+44 (0)20 7594 9846d.walsh

 
 
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Location

 

Faculty BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Sanz:1994:10.1111/j.1476-5381.1994.tb17019.x,
author = {Sanz, MJ and Weg, VB and Walsh, DT and Williams, TJ and Nourshargh, S},
doi = {10.1111/j.1476-5381.1994.tb17019.x},
journal = {Br J Pharmacol},
pages = {513--521},
title = {Differential effects of the PAF receptor antagonist UK-74,505 on neutrophil and eosinophil accumulation in guinea-pig skin.},
url = {http://dx.doi.org/10.1111/j.1476-5381.1994.tb17019.x},
volume = {113},
year = {1994}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - 1. The effect of the dihydropyridine, platelet activating factor (PAF) receptor antagonist, UK-74,505, on leucocyte accumulation and oedema formation in guinea-pig skin was investigated. The inflammatory reactions studied were elicited by exogenous mediators, a passive cutaneous anaphylactic (PCA) reaction and zymosan particles. 2. Leucocyte accumulation and oedema formation were measured as the local accumulation of i.v. administered 111In-labelled neutrophils or eosinophils together with 125I-labelled albumin. UK-74,505 was either administered i.v. or used to pretreat the radiolabelled leucocytes in vitro prior to their last wash and injection into recipient animals. 3. In vitro, UK-74,505 inhibited PAF-induced elevations in cytoplasmic levels of Ca2+ ([Ca2+]i) in fura-2-loaded guinea-pig neutrophils and eosinophils with IC50 values of 10(-9) M and 7 x 10(-9) M respectively. Neutrophils and eosinophils pretreated with 10(-7) M and 10(-6) M UK-74,505 respectively, and maintained at 37 degrees C, were unresponsive to PAF for the 4 h period investigated. 4. In vivo, using 2 h test periods, i.v. UK-74,505 (0.5 and 2.5 mg kg-1) inhibited the accumulation of 111In-neutrophils, 111In-eosinophils and oedema formation induced by intradermal PAF, but had no effect on responses elicited by leukotriene B4 (LTB4) and zymosan-activated plasma (ZAP, used as a source of C5a des Arg). UK-74,505 (2.5 mg kg-1) was also without an effect on response induced by a PCA reaction but significantly suppressed the 111In-eosinophil accumulation following the intradermal administration of zymosan particles. The 111In-neutrophil accumulation induced by zymosan particles was not, however, affected by UK-74,505. 5. In a second series of in vivo experiments, "'In-leucocytes were pretreated in vitro with UK-74,505 prior to their last wash and injection into recipient animals. Radiolabelled neutrophils, and eosinophils were pretreated with 10-7 M and 10-6 M UK-74,505 respectively, concentratio
AU  - Sanz,MJ
AU  - Weg,VB
AU  - Walsh,DT
AU  - Williams,TJ
AU  - Nourshargh,S
DO  - 10.1111/j.1476-5381.1994.tb17019.x
EP  - 521
PY  - 1994///
SN  - 0007-1188
SP  - 513
TI  - Differential effects of the PAF receptor antagonist UK-74,505 on neutrophil and eosinophil accumulation in guinea-pig skin.
T2  - Br J Pharmacol
UR  - http://dx.doi.org/10.1111/j.1476-5381.1994.tb17019.x
UR  - https://www.ncbi.nlm.nih.gov/pubmed/7834203
VL  - 113
ER  -
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