Imperial College London
Professor Dominic Withers

ProfessorDominicWithers

Faculty of Medicine, Institute of Clinical Sciences

Clinical Chair in Diabetes & Endocrinology
 
 
 
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Contact

 

d.withers

 
 
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Location

 

Hammersmith HospitalHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Jarvis:2020:10.1038/s41366-020-0595-6,
author = {Jarvis, S and Gethings, LA and Samanta, L and Pedroni, SMA and Withers, DJ and Gray, N and Plumb, RS and Winston, RML and Williamson, C and Bevan, CL},
doi = {10.1038/s41366-020-0595-6},
journal = {International Journal of Obesity},
pages = {1958--1969},
title = {High fat diet causes distinct aberrations in the testicular proteome},
url = {http://dx.doi.org/10.1038/s41366-020-0595-6},
volume = {44},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Diet has important effects on normal physiology and the potential deleterious effects of high fat diets and obesity on male reproductive health are being increasingly described. We conducted a histological review of the effects of chronic high fat (HF) diet (using a mouse model fed a 45% fat diet for 21 weeks) with a discovery proteomic study to assess for changes in the abundance of proteins in the testis. Mice on a HF diet became obese and developed glucose intolerance. Using mass spectrometry, we identify 102 proteins affected in the testis of obese mice. These included structural proteins important for the blood testis barrier (filamin A, FLNA), proteins involved in oxidative stress responses (spermatogenesis associated 20, SPATA-20) and lipid homoeostasis (sterol regulatory element-binding protein 2, SREBP2 and apolipoprotein A1, APOA1). In addition, an important regulator protein paraspeckle component 1, PSPC-1, which interacts with the androgen receptor was significantly downregulated. Proteomic data was validated using both Western blotting and immunostaining which confirmed and localised protein expression in both mouse and human testis using biopsy specimens. This study focused mainly on the abnormalities that occurred at the protein level and as a result, we have identified several candidate proteins and conducted pathway analysis around the effects of HF diet on the testis providing novel insights not previously described. Some of the identified targets could be targeted therapeutically and future work is directed in this area.
AU  - Jarvis,S
AU  - Gethings,LA
AU  - Samanta,L
AU  - Pedroni,SMA
AU  - Withers,DJ
AU  - Gray,N
AU  - Plumb,RS
AU  - Winston,RML
AU  - Williamson,C
AU  - Bevan,CL
DO  - 10.1038/s41366-020-0595-6
EP  - 1969
PY  - 2020///
SN  - 0307-0565
SP  - 1958
TI  - High fat diet causes distinct aberrations in the testicular proteome
T2  - International Journal of Obesity
UR  - http://dx.doi.org/10.1038/s41366-020-0595-6
UR  - https://www.nature.com/articles/s41366-020-0595-6
UR  - http://hdl.handle.net/10044/1/81267
VL  - 44
ER  -
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