Imperial College London
Alternate

Professor Denis Wright

Faculty of Natural Sciences, Department of Life Sciences

Emeritus Professor of Pest Management
 
 
 
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Contact

 

d.wright Website

 
 
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Location

 

Hamilton BuildingSilwood Park

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Summary

 

Publications

Citation

BibTex format

@article{Pinos:2020:10.3390/toxins12060409,
author = {Pinos, D and Chakroun, M and Millan-Leiva, A and Jurat-Fuentes, JL and Wright, DJ and Hernandez-Martinez, P and Ferre, J},
doi = {10.3390/toxins12060409},
journal = {Toxins},
pages = {1--12},
title = {Reduced membrane-bound alkaline phosphatase does not affect binding of Vip3Aa in aHeliothis virescensResistant colony},
url = {http://dx.doi.org/10.3390/toxins12060409},
volume = {12},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The Vip3Aa insecticidal protein from Bacillus thuringiensis (Bt) is produced by specific transgenic corn and cotton varieties for efficient control of target lepidopteran pests. The main threat to this technology is the evolution of resistance in targeted insect pests and understanding the mechanistic basis of resistance is crucial to deploy the most appropriate strategies for resistance management. In this work, we tested whether alteration of membrane receptors in the insect midgut might explain the >2000-fold Vip3Aa resistance phenotype in a laboratory-selected colony of Heliothis virescens (Vip-Sel). Binding of 125I-labeled Vip3Aa to brush border membrane vesicles (BBMV) from 3rd instar larvae from Vip-Sel was not significantly different from binding in the reference susceptible colony. Interestingly, BBMV from Vip-Sel larvae showed dramatically reduced levels of membrane-bound alkaline phosphatase (mALP) activity, which was further confirmed by a strong downregulation of the membrane-bound alkaline phosphatase 1 (HvmALP1) gene. However, the involvement of HvmALP1 as a receptor for the Vip3Aa protein was not supported by results from ligand blotting and viability assays with insect cells expressing HvmALP1.
AU  - Pinos,D
AU  - Chakroun,M
AU  - Millan-Leiva,A
AU  - Jurat-Fuentes,JL
AU  - Wright,DJ
AU  - Hernandez-Martinez,P
AU  - Ferre,J
DO  - 10.3390/toxins12060409
EP  - 12
PY  - 2020///
SN  - 2072-6651
SP  - 1
TI  - Reduced membrane-bound alkaline phosphatase does not affect binding of Vip3Aa in aHeliothis virescensResistant colony
T2  - Toxins
UR  - http://dx.doi.org/10.3390/toxins12060409
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000551188000056&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://www.mdpi.com/2072-6651/12/6/409
UR  - http://hdl.handle.net/10044/1/91212
VL  - 12
ER  -
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