Imperial College London

Professor David S. Rueda

Faculty of MedicineDepartment of Infectious Disease

Chair of Molecular and Cellular Medicine



+44 (0)20 3313 1604david.rueda Website




10N12ACommonwealth BuildingHammersmith Campus





Professor, Imperial College London, 2012 - present
Associate Professor, Wayne State Univerisity, 2011 - 12
Assistant Professor, Wayne State Univerisity, 2005 - 11
Postdoctoral Fellow, University of Michigan, 2001 - 05
Doctor ès Sciences, EPF Lausanne, 2001
Dipl. Chem. Eng., EPF Lausanne, 1997

The Single-Molecule Imaging Group develops and applies single-molecule imaging approaches to study how structural dynamics regulate fundamental biological processes involving proteins and nucleic acids across scales (from molecules to cells). We are particularly interested in elucidating various mechanistic aspects of DNA and RNA processing, such as chromatin structure and remodelling, DNA replication and repair, and RNA transcription, splicing and localization. Single-Molecule Microscopy reveals the structural dynamics of individual molecules, otherwise hidden in ensemble-averaged experiments, thus enabling us to directly observe key reaction intermediates, even when short-lived or at low levels. We currently focus on four main areas



Belan O, Moore G, Kaczmarczyk A, et al., 2021, Generation of versatile ss-dsDNA hybrid substrates for single-molecule analysis, Star Protocols, Vol:2, ISSN:2666-1667, Pages:100588-100588

Belan O, Barroso C, Kaczmarczyk A, et al., 2021, Single-molecule analysis reveals cooperative stimulation of Rad51 filament nucleation and growth by mediator proteins, Molecular Cell, Vol:81, ISSN:1097-2765, Pages:1058-1073.e7

Cawte AD, Unrau PJ, Rueda DS, 2020, Live cell imaging of single RNA molecules with fluorogenic Mango II arrays, Nature Communications, Vol:11, ISSN:2041-1723

Gutierrez-Escribano P, Newton MD, Llauro A, et al., 2019, A conserved ATP- and Scc2/4-dependent activity for cohesin in tethering DNA molecules, Science Advances, Vol:5, ISSN:2375-2548, Pages:1-15


Belan O, Barroso C, Kaczmarczyk A, et al., 2021, Single-Molecule Investigation of Rad-51 Presynaptic Filament Assembly and the Role of Mediator Proteins, CELL PRESS, Pages:33A-33A, ISSN:0006-3495

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