Imperial College London

Professor David S. Rueda

Faculty of MedicineDepartment of Infectious Disease

Chair of Molecular and Cellular Medicine
 
 
 
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Contact

 

+44 (0)20 3313 1604david.rueda Website

 
 
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Location

 

10N12ACommonwealth BuildingHammersmith Campus

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Summary

 

Summary

Professor, Imperial College London, 2012 - present
Associate Professor, Wayne State Univerisity, 2011 - 12
Assistant Professor, Wayne State Univerisity, 2005 - 11
Postdoctoral Fellow, University of Michigan, 2001 - 05
Doctor ès Sciences, EPF Lausanne, 2001
Dipl. Chem. Eng., EPF Lausanne, 1997


The Single-Molecule Imaging Group develops and applies single-molecule imaging approaches to study how structural dynamics regulate fundamental biological processes involving proteins and nucleic acids across scales (from molecules to cells). We are particularly interested in elucidating various mechanistic aspects of DNA and RNA processing, such as chromatin structure and remodelling, DNA replication and repair, and RNA transcription, splicing and localization. Single-Molecule Microscopy reveals the structural dynamics of individual molecules, otherwise hidden in ensemble-averaged experiments, thus enabling us to directly observe key reaction intermediates, even when short-lived or at low levels. We currently focus on four main areas

Publications

Journals

Newton MD, Fairbanks SD, Thomas JA, et al., 2021, A Minimal Load‐and‐Lock Ru II Luminescent DNA Probe, Angewandte Chemie, Vol:133, ISSN:0044-8249, Pages:21120-21127

Losito M, Smith QM, Newton MD, et al., 2021, Cas12a target search and cleavage on force-stretched DNA, Physical Chemistry Chemical Physics, ISSN:1463-9076

Newton MD, Fairbanks SD, Thomas JA, et al., 2021, A Minimal Load-and-Lock Ru-II Luminescent DNA Probe, Angewandte Chemie-international Edition, Vol:60, ISSN:1433-7851, Pages:20952-20959

Belan O, Moore G, Kaczmarczyk A, et al., 2021, Generation of versatile ss-dsDNA hybrid substrates for single-molecule analysis., Star Protoc, Vol:2

Belan O, Barroso C, Kaczmarczyk A, et al., 2021, Single-molecule analysis reveals cooperative stimulation of Rad51 filament nucleation and growth by mediator proteins, Molecular Cell, Vol:81, ISSN:1097-2765, Pages:1058-1073.e7

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