Summary
Professor, Imperial College London, 2012 - present
Associate Professor, Wayne State Univerisity, 2011 - 12
Assistant Professor, Wayne State Univerisity, 2005 - 11
Postdoctoral Fellow, University of Michigan, 2001 - 05
Doctor ès Sciences, EPF Lausanne, 2001
Dipl. Chem. Eng., EPF Lausanne, 1997
The Single-Molecule Imaging Group develops and applies single-molecule imaging approaches to study how structural dynamics regulate fundamental biological processes involving proteins and nucleic acids across scales (from molecules to cells). We are particularly interested in elucidating various mechanistic aspects of DNA and RNA processing, such as chromatin structure and remodelling, DNA replication and repair, and RNA transcription, splicing and localization. Single-Molecule Microscopy reveals the structural dynamics of individual molecules, otherwise hidden in ensemble-averaged experiments, thus enabling us to directly observe key reaction intermediates, even when short-lived or at low levels. We currently focus on four main areas.
Publications
Journals
Masood M, Ding Q, Cawte AD, et al., 2023, Genetic screening for anticancer genes highlights FBLN5 as a synthetic lethal partner of MYC, Cell Communication and Signaling, Vol:21, ISSN:1478-811X
Ploetz E, Ambrose B, Barth A, et al., 2023, A new twist on PIFE: photoisomerisation-related fluorescence enhancement., Methods Appl Fluoresc, Vol:12
Newton MD, Losito M, Smith QM, et al., 2023, Negative DNA supercoiling induces genome-wide Cas9 off-target activity., Mol Cell, Vol:83, Pages:3533-3545.e5
Han Z, Moore GA, Mitter R, et al., 2023, DNA-directed termination of RNA polymerase II transcription., Mol Cell, Vol:83, Pages:3253-3267.e7
Belan O, Greenhough L, Kuhlen L, et al., 2023, Visualization of direct and diffusion-assisted RAD51 nucleation by full-length human BRCA2 protein, Molecular Cell, Vol:83, ISSN:1097-2765, Pages:2925-2940.e8