Imperial College London
Portrait Picture

Professor David S. Rueda

Faculty of Medicine, Department of Infectious Disease

Chair in Molecular and Cellular Biophysics
 
 
 
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Contact

 

david.rueda Website

 
 
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Location

 

6.12DLMS BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Wilson:2019:10.1038/s41467-019-12007-w,
author = {Wilson, MD and Renault, L and Maskell, DP and Ghoneim, M and Pye, VE and Nans, A and Rueda, DS and Cherepanov, P and Costa, A},
doi = {10.1038/s41467-019-12007-w},
journal = {Nature Communications},
title = {Retroviral integration into nucleosomes through DNA looping and sliding along the histone octamer},
url = {http://dx.doi.org/10.1038/s41467-019-12007-w},
volume = {10},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Retroviral integrase can efficiently utilise nucleosomes for insertion of the reverse-transcribed viral DNA. In face of the structural constraints imposed by the nucleosomal structure, integrase gains access to the scissile phosphodiester bonds by lifting DNA off the histone octamer at the site of integration. To clarify the mechanism of DNA looping by integrase, we determined a 3.9 Å resolution structure of the prototype foamy virus intasome engaged with a nucleosome core particle. The structural data along with complementary single-molecule Förster resonance energy transfer measurements reveal twisting and sliding of the nucleosomal DNA arm proximal to the integration site. Sliding the nucleosomal DNA by approximately two base pairs along the histone octamer accommodates the necessary DNA lifting from the histone H2A-H2B subunits to allow engagement with the intasome. Thus, retroviral integration into nucleosomes involves the looping-and-sliding mechanism for nucleosomal DNA repositioning, bearing unexpected similarities to chromatin remodelers.
AU  - Wilson,MD
AU  - Renault,L
AU  - Maskell,DP
AU  - Ghoneim,M
AU  - Pye,VE
AU  - Nans,A
AU  - Rueda,DS
AU  - Cherepanov,P
AU  - Costa,A
DO  - 10.1038/s41467-019-12007-w
PY  - 2019///
SN  - 2041-1723
TI  - Retroviral integration into nucleosomes through DNA looping and sliding along the histone octamer
T2  - Nature Communications
UR  - http://dx.doi.org/10.1038/s41467-019-12007-w
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000485685900028&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/73585
VL  - 10
ER  -
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