Imperial College London
Portrait Picture

Professor David S. Rueda

Faculty of Medicine, Department of Infectious Disease

Chair in Molecular and Cellular Biophysics
 
 
 
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Contact

 

david.rueda Website

 
 
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Location

 

6.12DLMS BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Gutierrez-Escribano:2019:10.1126/sciadv.aay6804,
author = {Gutierrez-Escribano, P and Newton, MD and Llauro, A and Huber, J and Tanasie, L and Davy, J and Aly, I and Aramayo, R and Montoya, A and Kramer, H and Stigler, J and Rueda, DS and Aragon, L},
doi = {10.1126/sciadv.aay6804},
journal = {Science Advances},
pages = {1--15},
title = {A conserved ATP- and Scc2/4-dependent activity for cohesin in tethering DNA molecules},
url = {http://dx.doi.org/10.1126/sciadv.aay6804},
volume = {5},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Sister chromatid cohesion requires cohesin to act as a protein linker to hold chromatids together. How cohesin tethers chromatids remains poorly understood. We have used optical tweezers to visualize cohesin as it holds DNA molecules. We show that cohesin complexes tether DNAs in the presence of Scc2/Scc4 and ATP demonstrating a conserved activity from yeast to humans. Cohesin forms two classes of tethers: a “permanent bridge” resisting forces over 80 pN and a force-sensitive “reversible bridge.” The establishment of bridges requires physical proximity of dsDNA segments and occurs in a single step. “Permanent” cohesin bridges slide when they occur in trans, but cannot be removed when in cis. Therefore, DNAs occupy separate physical compartments in cohesin molecules. We finally demonstrate that cohesin tetramers can compact linear DNA molecules stretched by very low force (below 1 pN), consistent with the possibility that, like condensin, cohesin is also capable of loop extrusion.
AU  - Gutierrez-Escribano,P
AU  - Newton,MD
AU  - Llauro,A
AU  - Huber,J
AU  - Tanasie,L
AU  - Davy,J
AU  - Aly,I
AU  - Aramayo,R
AU  - Montoya,A
AU  - Kramer,H
AU  - Stigler,J
AU  - Rueda,DS
AU  - Aragon,L
DO  - 10.1126/sciadv.aay6804
EP  - 15
PY  - 2019///
SN  - 2375-2548
SP  - 1
TI  - A conserved ATP- and Scc2/4-dependent activity for cohesin in tethering DNA molecules
T2  - Science Advances
UR  - http://dx.doi.org/10.1126/sciadv.aay6804
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000499736100100&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://advances.sciencemag.org/content/5/11/eaay6804
UR  - http://hdl.handle.net/10044/1/88132
VL  - 5
ER  -
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