Imperial College London
Portrait Picture

Professor David S. Rueda

Faculty of Medicine, Department of Infectious Disease

Chair in Molecular and Cellular Biophysics
 
 
 
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Contact

 

david.rueda Website

 
 
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Location

 

6.12DLMS BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Newton:2021:10.1002/anie.202108077,
author = {Newton, MD and Fairbanks, SD and Thomas, JA and Rueda, DS},
doi = {10.1002/anie.202108077},
journal = {Angewandte Chemie International Edition},
pages = {20952--20959},
title = {A minimal load-and-lock Ru-II luminescent DNA probe},
url = {http://dx.doi.org/10.1002/anie.202108077},
volume = {60},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Threading intercalators bind DNA with high affinities. Here, we describe single-molecule studies on a cell-permeant luminescent dinuclear ruthenium(II) complex that has been previously shown to thread only into short, unstable duplex structures. Using optical tweezers and confocal microscopy, we show that this complex threads and locks into force-extended duplex DNA in a two-step mechanism. Detailed kinetic studies reveal that an individual stereoisomer of the complex exhibits the highest binding affinity reported for such a mono-intercalator. This stereoisomer better preserves the biophysical properties of DNA than the widely used SYTOX Orange. Interestingly, threading into torsionally constrained DNA decreases dramatically, but is rescued on negatively supercoiled DNA. Given the “light-switch” properties of this complex on binding DNA, it can be readily used as a long-lived luminescent label for duplex or negatively supercoiled DNA through a unique “load-and-lock” protocol.
AU  - Newton,MD
AU  - Fairbanks,SD
AU  - Thomas,JA
AU  - Rueda,DS
DO  - 10.1002/anie.202108077
EP  - 20959
PY  - 2021///
SN  - 1433-7851
SP  - 20952
TI  - A minimal load-and-lock Ru-II luminescent DNA probe
T2  - Angewandte Chemie International Edition
UR  - http://dx.doi.org/10.1002/anie.202108077
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000683791700001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://onlinelibrary.wiley.com/doi/10.1002/anie.202108077
UR  - http://hdl.handle.net/10044/1/93361
VL  - 60
ER  -
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