Imperial College London
Portrait Picture

Professor David S. Rueda

Faculty of Medicine, Department of Infectious Disease

Chair in Molecular and Cellular Biophysics
 
 
 
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Contact

 

david.rueda Website

 
 
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Location

 

6.12DLMS BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@inbook{Rueda:2021:10.1007/978-1-0716-1851-6_14,
author = {Rueda, D and Cawte, AD and Ino, H and Unrau, P},
booktitle = {Methods in Molecular Biology},
doi = {10.1007/978-1-0716-1851-6_14},
publisher = {Humana Press},
title = {Single-molecule RNA Imaging Using Mango II Arrays},
url = {http://dx.doi.org/10.1007/978-1-0716-1851-6_14},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - CHAP
AB  - In recent years, fluorogenic RNA aptamers, such as Spinach, Broccoli, Corn, Mango, Coral, and Pepper have gathered traction as an efficient alternative labeling strategy for background-free imaging of cellular RNAs. However, their application has been somewhat limited by relatively inefficient folding and fluorescent stability. With the recent advent of novel RNA-Mango variants which are improved in both fluorescence intensity and folding stability in tandem arrays, it is now possible to image RNAs with single-molecule sensitivity. Here we discuss the protocol for imaging Mango II tagged RNAs in both fixed and live cells.
AU  - Rueda,D
AU  - Cawte,AD
AU  - Ino,H
AU  - Unrau,P
DO  - 10.1007/978-1-0716-1851-6_14
PB  - Humana Press
PY  - 2021///
TI  - Single-molecule RNA Imaging Using Mango II Arrays
T1  - Methods in Molecular Biology
UR  - http://dx.doi.org/10.1007/978-1-0716-1851-6_14
UR  - https://link.springer.com/protocol/10.1007%2F978-1-0716-1851-6_14
UR  - http://hdl.handle.net/10044/1/93365
ER  -
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