Imperial College London
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Professor David S. Rueda

Faculty of Medicine, Department of Infectious Disease

Chair in Molecular and Cellular Biophysics
 
 
 
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Contact

 

david.rueda Website

 
 
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Location

 

6.12DLMS BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Rudan:2018:10.7554/eLife.35330,
author = {Rudan, M and Dib, PB and Musa, M and Kanunnikau, M and Sobocanec, S and Rueda, D and Warnecke, T and Krisko, A},
doi = {10.7554/eLife.35330},
journal = {eLife},
title = {Normal mitochondrial function in Saccharomyces cerevisiae has become dependent on inefficient splicing},
url = {http://dx.doi.org/10.7554/eLife.35330},
volume = {7},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Self-splicing introns are mobile elements that have invaded a number of highlyconserved genes in prokaryotic and organellar genomes. Here, we show that deletion of theseselfish elements from the Saccharomyces cerevisiae mitochondrial genome is stressful to the host.A strain without mitochondrial introns displays hallmarks of the retrograde response, with alteredmitochondrial morphology, gene expression and metabolism impacting growth and lifespan.Deletion of the complete suite of mitochondrial introns is phenocopied by overexpression of thesplicing factor Mss116. We show that, in both cases, abnormally efficient transcript maturationresults in excess levels of mature cob and cox1 host mRNA. Thus, inefficient splicing has becomean integral part of normal mitochondrial gene expression. We propose that the persistence of S.cerevisiae self-splicing introns has been facilitated by an evolutionary lock-in event, where the hostgenome adapted to primordial invasion in a way that incidentally rendered subsequent intron lossdeleterious.
AU  - Rudan,M
AU  - Dib,PB
AU  - Musa,M
AU  - Kanunnikau,M
AU  - Sobocanec,S
AU  - Rueda,D
AU  - Warnecke,T
AU  - Krisko,A
DO  - 10.7554/eLife.35330
PY  - 2018///
SN  - 2050-084X
TI  - Normal mitochondrial function in Saccharomyces cerevisiae has become dependent on inefficient splicing
T2  - eLife
UR  - http://dx.doi.org/10.7554/eLife.35330
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000429916200001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/59155
VL  - 7
ER  -
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