Imperial College London
Alternate

ProfessorEricAlton

Faculty of Medicine, National Heart & Lung Institute

Chair in Gene Therapy
 
 
 
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Contact

 

+44 (0)20 7594 7929e.alton

 
 
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Assistant

 

Miss Samia Soussi +44 (0)20 7594 7980

 
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Location

 

Emmanuel Kaye BuildingRoyal Brompton Campus

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Summary

 

Publications

Citation

BibTex format

@article{Bardin:2016:10.1002/ppul.23576,
author = {Bardin, E and Bolt, F and Cameron, S and Alton, E and Bush, A and Takats, Z and Davies, JC},
doi = {10.1002/ppul.23576},
journal = {Pediatric Pulmonology},
pages = {S194--S485},
title = {Metabolomic characterization of Pseudomonas aeruginosa isolates from cystic fibrosis patients using rapid evaporative ionisation mass spectrometry},
url = {http://dx.doi.org/10.1002/ppul.23576},
volume = {51},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Rapid evaporative ionization mass spectrometry (REIMS) is a new technique that has been shown to accurately classify yeast and bacteria. Thermal stress is applied onto bacterial colonies which results in evaporation and ionisation of metabolites and structural lipids. The mass spectrometer detects and identifies analytes through spectral database comparison (Strittmatter N, et al. Anal Chem. 2014;86:6555-62). We describe the application of REIMS to cystic fibrosis (CF)-related pathogens and show that the technology can identify P. aeruginosa with 100% accuracy. Differences were observed in the phospholipid and rhamnolipid range; derivatives of quorum sensing molecules (QSM) were also detected. These may provide useful biomarkers for a non-invasive diagnostic tool using ambient mass spectrometric (MS) approaches currently in development.
AU  - Bardin,E
AU  - Bolt,F
AU  - Cameron,S
AU  - Alton,E
AU  - Bush,A
AU  - Takats,Z
AU  - Davies,JC
DO  - 10.1002/ppul.23576
EP  - 485
PY  - 2016///
SN  - 8755-6863
SP  - 194
TI  - Metabolomic characterization of Pseudomonas aeruginosa isolates from cystic fibrosis patients using rapid evaporative ionisation mass spectrometry
T2  - Pediatric Pulmonology
UR  - http://dx.doi.org/10.1002/ppul.23576
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000384815300394&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/43723
VL  - 51
ER  -
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