Publications
47 results found
Soriani FM, Malavazi I, da Silva Ferreira ME, et al., 2008, Functional characterization of the <i>Aspergillus fumigatus</i> CRZ1 homologue, CrzA, MOLECULAR MICROBIOLOGY, Vol: 67, Pages: 1274-1291, ISSN: 0950-382X
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- Citations: 143
Schrettl M, Bignell E, Kragl C, et al., 2007, Distinct Roles for Intra- and Extracellular Siderophores during Aspergillus fumigatus Infection, PLoS Pathogens, Vol: 3
Siderophore biosynthesis by the highly lethal mould Aspergillus fumigatus is essential for virulence, but non-existent in humans, presenting a rare opportunity to strategize therapeutically against this pathogen. We have previously demonstrated that A. fumigatus excretes fusarinine C and triacetylfusarinine C to capture extracellular iron, and uses ferricrocin for hyphal iron storage. Here, we delineate pathways of intra- and extracellular siderophore biosynthesis and show that A. fumigatus synthesizes a developmentally regulated fourth siderophore, termed hydroxyferricrocin, employed for conidial iron storage. By inactivation of the nonribosomal peptide synthetase SidC, we demonstrate that the intracellular siderophores are required for germ tube formation, asexual sporulation, resistance to oxidative stress, catalase A activity, and virulence. Restoration of the conidial hydroxyferricrocin content partially rescues the virulence of the apathogenic siderophore null mutant DeltasidA, demonstrating an important role for the conidial siderophore during initiation of infection. Abrogation of extracellular siderophore biosynthesis following inactivation of the acyl transferase SidF or the nonribosomal peptide synthetase SidD leads to complete dependence upon reductive iron assimilation for growth under iron-limiting conditions, partial sensitivity to oxidative stress, and significantly reduced virulence, despite normal germ tube formation. Our findings reveal distinct cellular and disease-related roles for intra- and extracellular siderophores during mammalian Aspergillus infection.
Bignell E, Negrete-Urtasun S, Calcagno AM, et al., 2005, Virulence comparisons of <i>Aspergillus nidulans</i> mutants are confounded by the inflammatory response of p47<SUP><i>phox</i>-/-</SUP> mice, INFECTION AND IMMUNITY, Vol: 73, Pages: 5204-5207, ISSN: 0019-9567
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- Citations: 24
Calcagno AM, Bignell E, Rogers TR, et al., 2005, Candida glabrata Ste11 is involved in adaptation to hypertonic stress, maintenance of wild-type levels of filamentation and plays a role in virulence, MEDICAL MYCOLOGY, Vol: 43, Pages: 355-364, ISSN: 1369-3786
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- Citations: 16
Bignell E, Negrete-Urtasun S, Calcagno AM, et al., 2005, The <i>Aspergillus</i> pH-responsive transcription factor PacC regulates virulence, MOLECULAR MICROBIOLOGY, Vol: 55, Pages: 1072-1084, ISSN: 0950-382X
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- Citations: 86
Schrettl M, Bignell E, Kragl C, et al., 2004, Siderophore biosynthesis but not reductive iron assimilation is essential for <i>Aspergillus fumigates</i> virulence, JOURNAL OF EXPERIMENTAL MEDICINE, Vol: 200, Pages: 1213-1219, ISSN: 0022-1007
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- Citations: 393
Krappmann S, Reichard U, Bignell EM, et al., 2004, The Cross-Pathway Control system contributes to pathogenicity of <i>Aspergillus fumigatus</i>, Annual Meeting of the DGHM, Publisher: URBAN & FISCHER VERLAG, Pages: 193-193, ISSN: 1438-4221
Krappmann S, Bignell EM, Reichard U, et al., 2004, The Aspergillus fumigatus transcriptional activator CpcA contributes significantly to the virulence of this fungal pathogen, Mol Microbiol, Vol: 52, Pages: 785-799
Calcagno AM, Bignell E, Rogers TR, et al., 2004, Candida glabrata Ste20 is involved in maintaining cell wall integrity and adaptation to hypertonic stress, and is required for wild-type levels of virulence, YEAST, Vol: 21, Pages: 557-568, ISSN: 0749-503X
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- Citations: 22
Kamran M, Calcagno AM, Findon H, et al., 2004, Inactivation of transcription factor gene <i>ACE2</i> in the fungal pathogen <i>Candida glabrata</i> results in hypervirulence, EUKARYOTIC CELL, Vol: 3, Pages: 546-552, ISSN: 1535-9778
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- Citations: 55
Bignell E, Rogers T, 2004, Host recognition by fungal pathogens, Pathogenic fungi: Host interactions and emerging strategies for control, Editors: Sans-Blas, Calderone, Norfolk, Publisher: Caister Academic Press, Pages: 3-48, ISBN: 9780954246488
Calcagno AM, Bignell E, Warn P, et al., 2003, <i>Candida glabrata STE12</i> is required for wild-type levels of virulence and nitrogen starvation induced filamentation, MOLECULAR MICROBIOLOGY, Vol: 50, Pages: 1309-1318, ISSN: 0950-382X
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- Citations: 57
Espeso EA, Roncal T, Díez E, et al., 2000, On how a transcription factor can avoid its proteolytic activation in the absence of signal transduction, EMBO JOURNAL, Vol: 19, Pages: 2391-2391, ISSN: 0261-4189
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- Citations: 1
Espeso EA, Roncal T, Díez E, et al., 2000, On how a transcription factor can avoid its proteolytic activation in the absence of signal transduction, EMBO JOURNAL, Vol: 19, Pages: 719-728, ISSN: 0261-4189
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- Citations: 47
Mingot JM, Tilburn J, Diez E, et al., 1999, Specificity determinants of proteolytic processing of <i>Aspergillus</i> PacC transcription factor are remote from the processing site, and processing occurs in yeast if pH signalling is bypassed, MOLECULAR AND CELLULAR BIOLOGY, Vol: 19, Pages: 1390-1400, ISSN: 0270-7306
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- Citations: 64
Sarkar S, Caddick MX, Bignell E, et al., 1996, Regulation of gene expression by ambient pH in Aspergillus: Genes expressed at acid pH, BIOCHEMICAL SOCIETY TRANSACTIONS, Vol: 24, Pages: 360-363, ISSN: 0300-5127
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- Citations: 31
ARST HN, BIGNELL E, TILBURN J, 1994, 2 NEW GENES INVOLVED IN SIGNALING AMBIENT PH IN ASPERGILLUS-NIDULANS, MOLECULAR & GENERAL GENETICS, Vol: 245, Pages: 787-790, ISSN: 0026-8925
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- Citations: 87
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