Imperial College London
Alternate

ProfessorEmm MicDrakakis

Faculty of Engineering, Department of Bioengineering

Professor of Bio-Circuits and Systems
 
 
 
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Contact

 

e.drakakis Website

 
 
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Location

 

B207Bessemer BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Kim:2023:europace/euac176,
author = {Kim, MY and Nesbitt, J and Koutsoftidis, S and Brook, J and Pitcher, D and Cantwell, C and Handa, B and Jenkins, C and Houston, C and Rothery, S and Jothidasan, A and Perkins, J and Bristow, P and Linton, N and Drakakis, E and Peters, N and Chowdhury, R and Kanagaratnam, P and Ng, FS},
doi = {europace/euac176},
journal = {EP Europace},
pages = {726--738},
title = {Immunohistochemical characteristics of local sites that trigger atrial arrhythmias in response to high frequency stimulation},
url = {http://dx.doi.org/10.1093/europace/euac176},
volume = {25},
year = {2023}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Introduction: The response to high frequency stimulation (HFS) is used to locate putative sites of ganglionated plexuses (GPs), which are implicated in triggering atrial fibrillation (AF). Objective: To identify topological and immunohistochemical characteristics of presumed GP sites functionally identified by HFS. Methods: 63 atrial sites were tested with HFS in 4 Langendorff-perfused porcine hearts. A 3.5mm tip quadripolar ablation catheter was used to stimulate and deliver HFS to the left and right atrial epicardium, within the local atrial refractory period. Tissue samples from sites triggering atrial ectopy/AF (ET) sites and non-ET sites were stained with choline acetyl transferase (ChAT) and tyrosine hydroxylase (TH), for quantification of parasympathetic and sympathetic nerves, respectively. The average cross-sectional area (CSA) of nerves was also calculated.Results: Histomorphometry of 6 ET sites (9.5%) identified by HFS evoking at least a single atrial ectopic was compared with non-ET sites. All ET sites contained ChAT-immunoreactive (ChAT-IR) and/or TH-immunoreactive nerves (TH-IR). Nerve density was greater in ET sites compared to non-ET sites (nerves/cm2: 162.3 ±110.9 vs 69.65 ±72.48; p=0.047). Overall, TH-IR nerves had larger CSA than ChAT-IR nerves (µm2: 11,196 ± 35,141 vs 2,070 ± 5,841; p<0.0001), but in ET sites, TH-IR nerves were smaller than in non-ET sites (µm2: 6,021±14,586 vs 25,254 ± 61,499; p<0.001).Conclusions: ET sites identified by HFS contained higher density of smaller nerves than non-ET sites. Majority of these nerves were within the atrial myocardium. This has important clinical implications on devising an effective therapeutic strategy for targeting autonomic triggers of AF.
AU  - Kim,MY
AU  - Nesbitt,J
AU  - Koutsoftidis,S
AU  - Brook,J
AU  - Pitcher,D
AU  - Cantwell,C
AU  - Handa,B
AU  - Jenkins,C
AU  - Houston,C
AU  - Rothery,S
AU  - Jothidasan,A
AU  - Perkins,J
AU  - Bristow,P
AU  - Linton,N
AU  - Drakakis,E
AU  - Peters,N
AU  - Chowdhury,R
AU  - Kanagaratnam,P
AU  - Ng,FS
DO  - europace/euac176
EP  - 738
PY  - 2023///
SN  - 1099-5129
SP  - 726
TI  - Immunohistochemical characteristics of local sites that trigger atrial arrhythmias in response to high frequency stimulation
T2  - EP Europace
UR  - http://dx.doi.org/10.1093/europace/euac176
UR  - http://hdl.handle.net/10044/1/99873
VL  - 25
ER  -
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