Imperial College London
Alternate

ProfessorEmm MicDrakakis

Faculty of Engineering, Department of Bioengineering

Professor of Bio-Circuits and Systems
 
 
 
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Contact

 

e.drakakis Website

 
 
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Location

 

B207Bessemer BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Pedrigi:2016:10.1007/s10439-016-1750-z,
author = {Pedrigi, RM and Papadimitriou, KI and Kondiboyina, A and Sidhu, S and Chau, J and Patel, MB and Baeriswyl, DC and Drakakis, EM and Krams, R},
doi = {10.1007/s10439-016-1750-z},
journal = {Annals of Biomedical Engineering},
pages = {898--909},
title = {Disturbed cyclical stretch of endothelial cells promotes nuclear expression of the pro-atherogenic transcription factor NF-kappa B},
url = {http://dx.doi.org/10.1007/s10439-016-1750-z},
volume = {45},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Exposure of endothelial cells to low and multidirectional blood flow is known to promote a pro-atherogenic phenotype. The mechanics of the vessel wall is another important mechano-stimulus within the endothelial cell environment, but no study has examined whether changes in the magnitude and direction of cell stretch can be pro-atherogenic. Herein, we developed a custom cell stretching device to replicate the in vivo stretch environment of the endothelial cell and examined whether low and multidirectional stretch promote nuclear translocation of NF-κB. A fluid–structure interaction model of the device demonstrated a nearly uniform strain within the region of cell attachment and a negligible magnitude of shear stress due to cyclical stretching of the cells in media. Compared to normal cyclical stretch, a low magnitude of cyclical stretch or no stretch caused increased expression of nuclear NF-κB (p = 0.09 and p < 0.001, respectively). Multidirectional stretch also promoted significant nuclear NF-κB expression, comparable to the no stretch condition, which was statistically higher than the low (p < 0.001) and normal (p < 0.001) stretch conditions. This is the first study to show that stretch conditions analogous to atherogenic blood flow profiles can similarly promote a pro-atherogenic endothelial cell phenotype, which supports a role for disturbed vessel wall mechanics as a pathological cell stimulus in the development of advanced atherosclerotic plaques.
AU  - Pedrigi,RM
AU  - Papadimitriou,KI
AU  - Kondiboyina,A
AU  - Sidhu,S
AU  - Chau,J
AU  - Patel,MB
AU  - Baeriswyl,DC
AU  - Drakakis,EM
AU  - Krams,R
DO  - 10.1007/s10439-016-1750-z
EP  - 909
PY  - 2016///
SN  - 1573-9686
SP  - 898
TI  - Disturbed cyclical stretch of endothelial cells promotes nuclear expression of the pro-atherogenic transcription factor NF-kappa B
T2  - Annals of Biomedical Engineering
UR  - http://dx.doi.org/10.1007/s10439-016-1750-z
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000398737100004&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/45804
VL  - 45
ER  -
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