Imperial College London
Alternate

DrStathisGiotis

Faculty of Medicine, Department of Infectious Disease

Honorary Senior Research Fellow
 
 
 
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Contact

 

+44 (0)20 7594 9057e.giotis

 
 
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Location

 

Medical SchoolSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Mariatulqabtiah:2019,
author = {Mariatulqabtiah, AR and Majid, NN and Giotis, ES and Omar, AR and Skinner, MA},
journal = {Asia-Pacific Journal of Molecular Biology and Biotechnology},
pages = {84--94},
title = {Inoculation of fowlpox viruses coexpressing avian influenza H5 and chicken IL-15 cytokine gene stimulates diverse host immune responses},
volume = {27},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - © 2019, University of Malaya. All rights reserved. Fowlpox virus (FWPV) has been used as a recombinant vaccine vector to express antigens from several important avian pathogens. Attempts have been made to improve vaccine strains induced-host immune responses by coexpressing cytokines. This study describes the construction of recombinant FWPV (rFWPV) strain FP9 and immunological responses in specific-pathogen-free (SPF) chickens, coexpressing avian influenza virus (AIV) H5 of A/Chicken/Malaysia/5858/2004, and chicken IL-15 cytokine genes. Expression of H5 (50 kD) was confirmed by western blotting. Anti-H5 antibodies, which were measured by the haemagglutinin inhibition test, were at the highest levels at Week 3 post-inoculation in both rFWPV/H5-and rFWPV/H5/IL-15-vaccinated chickens, but decreased to undetectable levels from Week 5 onwards. CD3+/CD4+ or CD3+/CD8+T cell populations, assessed using flow cytometry, were significantly increased in both WT FP9-and rFWPV/H5-vaccinated chickens and were also higher than in rFWPV/H5/IL-15-vaccinated chickens, at Week 2. Gene expression analysis using real time quantitative polymerase chain reaction (qPCR) demonstrated upregulation of IL-15 expression in all vaccinated groups with rFWPV/H5/IL-15 having the highest fold change, at day 2 (117±51.53). Despite showing upregulation, fold change values of the IL-18 expression were below 1.00 for all vaccinated groups at day 2, 4 and 6. This study shows successful construction of rFWPV/H5 co-expressing IL-15, with modified immunogenicity upon inoculation into SPF chickens.
AU  - Mariatulqabtiah,AR
AU  - Majid,NN
AU  - Giotis,ES
AU  - Omar,AR
AU  - Skinner,MA
EP  - 94
PY  - 2019///
SN  - 0128-7451
SP  - 84
TI  - Inoculation of fowlpox viruses coexpressing avian influenza H5 and chicken IL-15 cytokine gene stimulates diverse host immune responses
T2  - Asia-Pacific Journal of Molecular Biology and Biotechnology
VL  - 27
ER  -
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