Imperial College London
Alternate

DrStathisGiotis

Faculty of Medicine, Department of Infectious Disease

Honorary Senior Research Fellow
 
 
 
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Contact

 

+44 (0)20 7594 9057e.giotis

 
 
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Location

 

Medical SchoolSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Dulwich:2020:10.3389/fcimb.2020.00315,
author = {Dulwich, KL and Asfor, A and Gray, A and Giotis, ES and Skinner, MA and Broadbent, AJ},
doi = {10.3389/fcimb.2020.00315},
journal = {Frontiers in Cellular and Infection Microbiology},
title = {The stronger downregulation of in vitro and in vivo innate antiviral responses by a very virulent strain of Infectious Bursal Disease Virus (IBDV), compared to a classical strain, is mediated, in part, by the VP4 protein},
url = {http://dx.doi.org/10.3389/fcimb.2020.00315},
volume = {10},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - IBDV is economically important to the poultry industry. Very virulent (vv) strains cause higher mortality rates than other strains for reasons that remain poorly understood. In order to provide more information on IBDV disease outcome, groups of chickens (n = 18) were inoculated with the vv strain, UK661, or the classical strain, F52/70. Birds infected with UK661 had a lower survival rate (50%) compared to F52/70 (80%). There was no difference in peak viral replication in the bursa of Fabricius (BF), but the expression of chicken IFNα, IFNβ, MX1, and IL-8 was significantly lower in the BF of birds infected with UK661 compared to F52/70 (p < 0.05) as quantified by RTqPCR, and this trend was also observed in DT40 cells infected with UK661 or F52/70 (p < 0.05). The induction of expression of type I IFN in DF-1 cells stimulated with polyI:C (measured by an IFN-β luciferase reporter assay) was significantly reduced in cells expressing ectopic VP4 from UK661 (p < 0.05), but was higher in cells expressing ectopic VP4 from F52/70. Cells infected with a chimeric recombinant IBDV carrying the UK661-VP4 gene in the background of PBG98, an attenuated vaccine strain that induces high levels of innate responses (PBG98-VP4UK661) also showed a reduced level of IFNα and IL-8 compared to cells infected with a chimeric virus carrying the F52/70-VP4 gene (PBG98-VP4F52/70) (p < 0.01), and birds infected with PBG98-VP4UK661 also had a reduced expression of IFNα in the BF compared to birds infected with PBG98-VP4F52/70 (p < 0.05). Taken together, these data demonstrate that UK661 induced the expression of lower levels of anti-viral type I IFN and proinflammatory genes than the classical strain in vitro and in vivo and this was, in part, due to strain-dependent differences in the VP4 protein.
AU  - Dulwich,KL
AU  - Asfor,A
AU  - Gray,A
AU  - Giotis,ES
AU  - Skinner,MA
AU  - Broadbent,AJ
DO  - 10.3389/fcimb.2020.00315
PY  - 2020///
SN  - 2235-2988
TI  - The stronger downregulation of in vitro and in vivo innate antiviral responses by a very virulent strain of Infectious Bursal Disease Virus (IBDV), compared to a classical strain, is mediated, in part, by the VP4 protein
T2  - Frontiers in Cellular and Infection Microbiology
UR  - http://dx.doi.org/10.3389/fcimb.2020.00315
UR  - https://www.ncbi.nlm.nih.gov/pubmed/32582573
UR  - http://hdl.handle.net/10044/1/80715
VL  - 10
ER  -
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