Imperial College London
Alternate

DrStathisGiotis

Faculty of Medicine, Department of Infectious Disease

Honorary Senior Research Fellow
 
 
 
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Contact

 

+44 (0)20 7594 9057e.giotis

 
 
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Location

 

Medical SchoolSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Zhang:2022:10.1101/2022.02.02.478897,
author = {Zhang, Z and Penn, R and Barclay, WS and Giotis, ES},
doi = {10.1101/2022.02.02.478897},
title = {Primary macrophages exhibit a modest inflammatory response early in SARS-CoV-2 infection},
url = {http://dx.doi.org/10.1101/2022.02.02.478897},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - <jats:title>Abstract</jats:title><jats:p>Involvement of macrophages in the SARS-CoV-2-associated cytokine storm, the excessive secretion of inflammatory/anti-viral factors leading to the acute respiratory distress syndrome (ARDS) in COVID-19 patients, is unclear. In this study, we sought to characterize the interplay between the virus and primary human monocyte-derived macrophages (MDM). MDM were stimulated with recombinant IFN-α and/or infected with either live or UV-inactivated SARS-CoV-2 or with two reassortant influenza viruses containing external genes from the H1N1 PR8 strain and heterologous internal genes from a highly pathogenic avian H5N1 or a low pathogenic human seasonal H1N1 strain. Virus replication was monitored by qRT-PCR for the<jats:italic>E</jats:italic>viral gene for SARS-CoV-2 or<jats:italic>M</jats:italic>gene for influenza and TCID<jats:sub>50</jats:sub>or plaque assay, and cytokine levels were assessed semiquantitatively with qRT-PCR and a proteome cytokine array. We report that MDM are not susceptible to SARS-CoV-2 whereas both influenza viruses replicated in MDM, albeit abortively. We observed a modest cytokine response in SARS-CoV-2 infected MDM with notable absence of IFN-β induction, which was instead strongly induced by the influenza viruses. Pre-treatment of MDM with IFN-α enhanced proinflammatory cytokine expression upon infection. Together, the findings concur that the hyperinflammation observed in SARS-CoV-2 infection is not driven by macrophages.</jats:p>
AU  - Zhang,Z
AU  - Penn,R
AU  - Barclay,WS
AU  - Giotis,ES
DO  - 10.1101/2022.02.02.478897
PY  - 2022///
TI  - Primary macrophages exhibit a modest inflammatory response early in SARS-CoV-2 infection
UR  - http://dx.doi.org/10.1101/2022.02.02.478897
ER  -
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