Imperial College London
Alternate

DrStathisGiotis

Faculty of Medicine, Department of Infectious Disease

Honorary Senior Research Fellow
 
 
 
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Contact

 

+44 (0)20 7594 9057e.giotis

 
 
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Location

 

Medical SchoolSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Zhang:2022:10.3390/v14020441,
author = {Zhang, Z and Penn, R and Barclay, WS and Giotis, E},
doi = {10.3390/v14020441},
journal = {Viruses},
pages = {1--10},
title = {Naïve human macrophages are refractory to SARS-CoV-2 infection and exhibit a modest inflammatory response early in infection},
url = {http://dx.doi.org/10.3390/v14020441},
volume = {14},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Involvement of macrophages in the SARS-CoV-2-associated cytokine storm, the excessive secretion of inflammatory/anti-viral factors leading to the acute respiratory distress syndrome (ARDS) in COVID-19 patients, is unclear. In this study, we sought to characterize the interplay between the virus and primary human monocyte-derived macrophages (MDM). MDM were stimulated with recombinant IFN- and/or infected with either live or UV-inactivated SARS-CoV- 2 or with two reassortant influenza viruses containing external genes from the H1N1 PR8 strain and heterologous internal genes from a highly pathogenic avian H5N1 or a low pathogenic human seasonal H1N1 strain. Virus replication was monitored by qRT-PCR for the E viral gene for SARS- CoV-2 or M gene for influenza and TCID50 or plaque assay, and cytokine levels were assessed semiquantitatively with qRT-PCR and a proteome cytokine array. We report that MDM are not susceptible to SARS-CoV-2 whereas both influenza viruses replicated in MDM, albeit abortively. We observed a modest cytokine response in SARS-CoV-2 exposed MDM with notable absence of IFN-β induction, which was instead strongly induced by the influenza viruses. Pre-treatment of MDM with IFN-α enhanced proinflammatory cytokine expression upon exposure to virus. Together, the findings concur that the hyperinflammation observed in SARS-CoV-2 infection is not driven by macrophages.
AU  - Zhang,Z
AU  - Penn,R
AU  - Barclay,WS
AU  - Giotis,E
DO  - 10.3390/v14020441
EP  - 10
PY  - 2022///
SN  - 1999-4915
SP  - 1
TI  - Naïve human macrophages are refractory to SARS-CoV-2 infection and exhibit a modest inflammatory response early in infection
T2  - Viruses
UR  - http://dx.doi.org/10.3390/v14020441
UR  - https://www.mdpi.com/1999-4915/14/2/441
UR  - http://hdl.handle.net/10044/1/94815
VL  - 14
ER  -
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