Imperial College London
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Dr. Elita Jauneikaite

Faculty of Medicine, School of Public Health

Advanced Research Fellow
 
 
 
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Contact

 

e.jauneikaite

 
 
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Location

 

Sir Michael Uren HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Taylor:2022:10.1016/j.ijantimicag.2022.106550,
author = {Taylor, E and Jauneikaite, E and Sriskandan, S and Woodford, N and Hopkins, K},
doi = {10.1016/j.ijantimicag.2022.106550},
journal = {International Journal of Antimicrobial Agents},
title = {Detection and characterisation of 16S rRNA methyltransferase-producing Pseudomonas aeruginosa from the UK and Republic of Ireland from 2003-2015},
url = {http://dx.doi.org/10.1016/j.ijantimicag.2022.106550},
volume = {59},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - 16S rRNA methyltransferase (16S RMTase) genes confer high-level aminoglycoside resistance, reducing treatment options for multidrug-resistant Gram-negative bacteria. Pseudomonas aeruginosa isolates (n = 221) exhibiting high-level pan-aminoglycoside resistance (amikacin, gentamicin and tobramycin MICs ≥64, ≥32 and ≥32 mg/L, respectively) were screened for 16S RMTase genes to determine their occurrence among isolates submitted to a national reference laboratory from December 2003 to December 2015. 16S RMTase genes were identified using two multiplex PCRs, and whole-genome sequencing (WGS) was used to identify other antibiotic resistance genes, sequence types (STs) and the genetic environment of 16S RMTase genes. 16S RMTase genes were found in 8.6% (19/221) of isolates, with rmtB4 (47.4%; 9/19) being most common, followed by rmtD3 (21.1%; 4/19), rmtF2 (15.8%; 3/19) and single isolates harbouring rmtB1, rmtC and rmtD1. Carbapenemase genes were found in 89.5% (17/19) of 16S RMTase-positive isolates, with blaVIM (52.9%; 9/17) being most common. 16S RMTase genes were found in ‘high-risk’ clones known to harbour carbapenemase genes (ST233, ST277, ST357, ST654 and ST773). Analysis of the genetic environment of 16S RMTase genes identified that IS6100 was genetically linked to rmtB1; IS91 to rmtB4, rmtC or rmtD3; ISCR14 to rmtD1; and rmtF2 was linked to Tn3, IS91 or Tn1721. Although 16S RMTase genes explained only 8.6% of pan-aminoglycoside resistance in the P. aeruginosa isolates studied, the association of 16S RMTase genes with carbapenemase-producers and ‘high-risk’ clones highlights that continued surveillance is required to monitor spread as well as the importance of suppressing the emergence of dually-resistant clones in hospital settings.
AU  - Taylor,E
AU  - Jauneikaite,E
AU  - Sriskandan,S
AU  - Woodford,N
AU  - Hopkins,K
DO  - 10.1016/j.ijantimicag.2022.106550
PY  - 2022///
SN  - 0924-8579
TI  - Detection and characterisation of 16S rRNA methyltransferase-producing Pseudomonas aeruginosa from the UK and Republic of Ireland from 2003-2015
T2  - International Journal of Antimicrobial Agents
UR  - http://dx.doi.org/10.1016/j.ijantimicag.2022.106550
UR  - http://hdl.handle.net/10044/1/95739
VL  - 59
ER  -
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