Imperial College London
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Dr. Elita Jauneikaite

Faculty of Medicine, School of Public Health

Advanced Research Fellow
 
 
 
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Contact

 

e.jauneikaite

 
 
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Location

 

Sir Michael Uren HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Zhi:2022:10.1101/2022.12.18.520871,
author = {Zhi, X and Li, HK and Li, H and Loboda, Z and Charles, S and Vieira, A and Huse, K and Jauneikaite, E and Coelho, J and Lamagni, T and Sriskandan, S},
doi = {10.1101/2022.12.18.520871},
title = {Ongoing emergence of M1<sub>UK</sub>lineage among invasive group A streptococcus isolates in 2020 and use of allele-specific PCR},
url = {http://dx.doi.org/10.1101/2022.12.18.520871},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - <jats:title>Summary</jats:title><jats:sec><jats:title>Background</jats:title><jats:p>An increasing burden of invasive group A streptococcal infections is reported in multiple countries, notably England, where scarlet fever cases are also abundant. In England, increased scarlet fever and invasive infections have been associated with emergence of a sublineage of<jats:italic>emm</jats:italic>1<jats:italic>Streptococcus pyogenes</jats:italic>that expresses increased SpeA scarlet fever erythrogenic toxin. Wider surveillance for toxigenic<jats:italic>Streptococcus pyogenes</jats:italic>lineage M1<jats:sub>UK</jats:sub>is much needed however, to date, lineage assignment has required genome sequencing limiting surveillance to those centres with access to such facilities.</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>To circumvent the requirement for genome sequencing, an allele-specific PCR was developed to distinguish M1<jats:sub>UK</jats:sub>from other<jats:italic>emm</jats:italic>1 strains. Additional PCR assays were developed to distinguish M1<jats:sub>UK</jats:sub>from two intermediate lineages that were detected previously. The assay was evaluated using DNA from genome-sequenced upper respiratory tract<jats:italic>emm</jats:italic>1<jats:italic>S. pyogenes</jats:italic>strains and a further set of 16 genome-sequenced invasive<jats:italic>S. pyogenes</jats:italic>isolates that included the two intermediate lineages. The assay was then applied to DNA from all 305 invasive<jats:italic>emm</jats:italic>1 isolates that had been submitted to the reference laboratory in the one pear period Jan 1-Dec 31 2020, in order to assign lineage.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>The allele sp
AU  - Zhi,X
AU  - Li,HK
AU  - Li,H
AU  - Loboda,Z
AU  - Charles,S
AU  - Vieira,A
AU  - Huse,K
AU  - Jauneikaite,E
AU  - Coelho,J
AU  - Lamagni,T
AU  - Sriskandan,S
DO  - 10.1101/2022.12.18.520871
PY  - 2022///
TI  - Ongoing emergence of M1<sub>UK</sub>lineage among invasive group A streptococcus isolates in 2020 and use of allele-specific PCR
UR  - http://dx.doi.org/10.1101/2022.12.18.520871
ER  -
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