Imperial College London
Alternate

DrErikaRosivatz

Faculty of Natural Sciences, Department of Chemistry

Departmental Operations Manager
 
 
 
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Contact

 

+44 (0)20 7594 5718e.rosivatz Website

 
 
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Location

 

G02Molecular Sciences Research HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Kremer:2003:carcin/bgg148,
author = {Kremer, M and Quintanilla-Martinez, L and Fuchs, M and Gamboa-Dominguez, A and Haye, S and Kalthoff, H and Rosivatz, E and Hermannstadter, C and Busch, R and Hofler, H and Luber, B},
doi = {carcin/bgg148},
journal = {Carcinogenesis},
pages = {1879--1886},
title = {Influence of tumor-associated E-cadherin mutations on tumorigenicity and metastasis},
url = {http://dx.doi.org/10.1093/carcin/bgg148},
volume = {24},
year = {2003}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - In this study, we investigated whether tumor-associated E-cadherin mutations impair the tumor-suppressive function of the cell adhesion molecule and influence metastasis formation in a severe combined immunodeficiency mouse model. The investigated E-cadherin mutations were in frame deletions of exons 8 (del 8) or 9 (del 9) and a point mutation in exon 8 (p8). Transfected human MDA-MB-435S carcinoma cells stably expressing wild-type (wt) or mutant E-cadherin were injected into the mouse mammary fat pad. Mice transplanted with wt E-cadherin transfectants developed significantly smaller tumors than animals transplanted with the E-cadherin-negative parental cell line. Animals transplanted with del 9 or p8 E-cadherin transfectants produced medium size tumors, indicating that these mutations impair the tumor-suppressive function of E-cadherin. In contrast, mice transplanted with del 8 E-cadherin transfectants developed tumors of approximately the same sizes as animals transplanted with wt E-cadherin expressing cells. Lung metastases were induced by all cell lines without significant differences. Immunohistochemical analysis of E-cadherin expression in the tumors revealed a heterogeneous staining pattern, indicating loss or down-regulation of E-cadherin in some tumor cells. Metastases were completely negative for E-cadherin. Our data suggest that the type of mutation determines whether the tumor-suppressive function of E-cadherin is impaired
AU  - Kremer,M
AU  - Quintanilla-Martinez,L
AU  - Fuchs,M
AU  - Gamboa-Dominguez,A
AU  - Haye,S
AU  - Kalthoff,H
AU  - Rosivatz,E
AU  - Hermannstadter,C
AU  - Busch,R
AU  - Hofler,H
AU  - Luber,B
DO  - carcin/bgg148
EP  - 1886
PY  - 2003///
SP  - 1879
TI  - Influence of tumor-associated E-cadherin mutations on tumorigenicity and metastasis
T2  - Carcinogenesis
UR  - http://dx.doi.org/10.1093/carcin/bgg148
UR  - pm:12949051
VL  - 24
ER  -
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