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@article{Fricke:2003:10.1002/ijc.10879,
author = {Fricke, E and Keller, G and Becker, I and Rosivatz, E and Schott, C and Plaschke, S and Rudelius, M and Hermannstadter, C and Busch, R and Hofler, H and Becker, KF and Luber, B},
doi = {10.1002/ijc.10879},
journal = {Int.J.Cancer},
pages = {60--65},
title = {Relationship between E-cadherin gene mutation and p53 gene mutation, p53 accumulation, Bcl-2 expression and Ki-67 staining in diffuse-type gastric carcinoma},
url = {http://dx.doi.org/10.1002/ijc.10879},
volume = {104},
year = {2003}
}

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TY  - JOUR
AB  - E-cadherin mutations are found in 50% of diffuse-type gastric carcinoma, but not in intestinal gastric carcinoma. Because cell-cell adhesion mediated by E-cadherin plays an important role in epithelial cell survival, E-cadherin mutations could alter the apoptotic behavior of tumor cells. p53 and Bcl-2 family members are also important regulators of cellular apoptosis. This is the first study that investigates the relationship between E-cadherin gene mutation and p53 gene mutation, p53 accumulation, Bcl-2 expression, and Ki-67 expression in diffuse-type gastric carcinoma (24 cases, E-cadherin mutation status: wild-type in 8 patients and mutant in 16 patients). The mutation status of exons 5-8 of p53 was analyzed by denaturing high pressure liquid chromatography (DHPLC) in formalin-fixed, paraffin-embedded tumor sections, followed by direct sequencing of cases with aberrant chromatographic patterns. p53 mutations were found in 1 of 8 tumors without E-cadherin mutation (12.5%) and in 1 of 16 tumors with E-cadherin mutation (6.3%), a difference that was not statistically significant (p = 1.00). p53 accumulation was found in 8 of 24 tumors (33.3%) by immunohistochemical staining. p53 accumulation was significantly more frequent in tumors without E-cadherin mutations (5 of 8 tumors, 62.5%) than in gastric carcinoma tissues with E-cadherin mutations (3 of 16 tumors, 18.8%, p = 0.03). Bcl-2 staining was not observed in gastric carcinoma cells without E-cadherin mutations, but was detectable in 5 of 16 tumors with E-cadherin mutations (31.3%), a difference that was not statistically significant (p = 0.13). No relationship was observed between Ki-67 staining and the E-cadherin mutation status (p = 1.00). These data suggest that the presence of E-cadherin mutations can significantly alter the accumulation of the apoptosis-regulating p53 protein, whereas no correlation with the p53 mutation status or with Ki-67 staining was observed
AU  - Fricke,E
AU  - Keller,G
AU  - Becker,I
AU  - Rosivatz,E
AU  - Schott,C
AU  - Plaschke,S
AU  - Rudelius,M
AU  - Hermannstadter,C
AU  - Busch,R
AU  - Hofler,H
AU  - Becker,KF
AU  - Luber,B
DO  - 10.1002/ijc.10879
EP  - 65
PY  - 2003///
SP  - 60
TI  - Relationship between E-cadherin gene mutation and p53 gene mutation, p53 accumulation, Bcl-2 expression and Ki-67 staining in diffuse-type gastric carcinoma
T2  - Int.J.Cancer
UR  - http://dx.doi.org/10.1002/ijc.10879
UR  - pm:12532420
VL  - 104
ER  -

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