Imperial College London
Alternate

DrErikaRosivatz

Faculty of Natural Sciences, Department of Chemistry

Departmental Operations Manager
 
 
 
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Contact

 

+44 (0)20 7594 5718e.rosivatz Website

 
 
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Location

 

G02Molecular Sciences Research HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Rosivatz:2009:10.1007/978-1-60327-115-8_14,
author = {Rosivatz, E and Woscholski, R},
doi = {10.1007/978-1-60327-115-8_14},
journal = {Methods Mol.Biol.},
pages = {213--222},
title = {Measurement of PTEN activity in vivo by imaging phosphorylated Akt},
url = {http://dx.doi.org/10.1007/978-1-60327-115-8_14},
volume = {462},
year = {2009}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - This chapter describes an indirect approach to measure PTEN's lipid phosphatase activity in vivo. PTEN counteracts phosphatidylinositol 3-kinase action in dephosphorylating 3-phosphorylated phosphoinositides. Therefore, PtdIns(3,4,5)P3-dependent activation and phosphorylation of the survival kinase Akt can be used as readout for cellular PTEN activity. Here we have outlined a detailed procedure employing a phosphoserine-specific anti-Akt antibody to examine the content of phosphorylated Akt by immunofluorescence and its dependence on PTEN activity
AU  - Rosivatz,E
AU  - Woscholski,R
DO  - 10.1007/978-1-60327-115-8_14
EP  - 222
PY  - 2009///
SP  - 213
TI  - Measurement of PTEN activity in vivo by imaging phosphorylated Akt
T2  - Methods Mol.Biol.
UR  - http://dx.doi.org/10.1007/978-1-60327-115-8_14
UR  - pm:19160672
VL  - 462
ER  -
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