Imperial College London

MissEllasiaTan

Faculty of Natural SciencesDepartment of Physics

Research Postgraduate
 
 
 
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Contact

 

e.tan17

 
 
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Location

 

H724Huxley BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Tan:2020:10.1002/bit.27187,
author = {Tan, E and Pappa, A-M and Pitsalidis, C and Nightingale, J and Wood, S and Castro, FA and Owens, RM and Kim, J-S},
doi = {10.1002/bit.27187},
journal = {Biotechnology and Bioengineering},
pages = {291--299},
title = {A highly sensitive molecular structural probe applied to in-situ biosensing of metabolites using PEDOT:PSS},
url = {http://dx.doi.org/10.1002/bit.27187},
volume = {117},
year = {2020}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - A large amount of research within organic biosensors is dominated by organic electrochemical transistors (OECTs) that use conducting polymers such as poly(3,4-ethylene dioxythiophene doped with poly(styrenesulfonate) (PEDOT:PSS). Despite the recent advances in OECT-based biosensors, the sensing is solely reliant on the amperometric detection of the bioanalytes. This is typically accompanied by large undesirable parasitic electrical signals from the electroactive components in the electrolyte. Herein, we present the use of in-situ resonance Raman spectroscopy to probe subtle molecular structural changes of PEDOT:PSS associated with its doping level. We demonstrate how such doping level changes of PEDOT:PSS can be used, for the first time, on operational OECTs for sensitive and selective metabolite sensing whilst simultaneously performing amperometric detection of the analyte. We test the sensitivity by molecularly sensing a lowest glucose concentration of 0.02 mM in phosphate buffered saline (PBS) solution. By changing the electrolyte to cell culture media, the selectivity of in-situ resonance Raman spectroscopy is emphasized as it remains unaffected by other electroactive components in the electrolyte. The application of this molecular structural probe highlights the importance of developing biosensing probes that benefit from high sensitivity of the material's structural and electrical properties whilst being complimentary with the electronic methods of detection.
AU - Tan,E
AU - Pappa,A-M
AU - Pitsalidis,C
AU - Nightingale,J
AU - Wood,S
AU - Castro,FA
AU - Owens,RM
AU - Kim,J-S
DO - 10.1002/bit.27187
EP - 299
PY - 2020///
SN - 0006-3592
SP - 291
TI - A highly sensitive molecular structural probe applied to in-situ biosensing of metabolites using PEDOT:PSS
T2 - Biotechnology and Bioengineering
UR - http://dx.doi.org/10.1002/bit.27187
UR - https://www.ncbi.nlm.nih.gov/pubmed/31589342
UR - https://onlinelibrary.wiley.com/doi/full/10.1002/bit.27187
UR - http://hdl.handle.net/10044/1/74126
VL - 117
ER -