Imperial College London

Dr Evangelos Triantafyllou

Faculty of MedicineDepartment of Metabolism, Digestion and Reproduction

Advanced Research Fellow
 
 
 
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Contact

 

e.triantafyllou Website

 
 
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Location

 

Queen Elizabeth the Queen Mother Wing (QEQM)St Mary's Campus

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Summary

 

Publications

Publication Type
Year
to

53 results found

Artru F, McPhail MJW, Triantafyllou E, Trovato FMet al., 2022, Lipids in liver failure syndromes: a focus on eicosanoids, specialized pro-resolvinglipid mediators and lysophospholipids., Frontiers in Immunology, Vol: 13, Pages: 1-21, ISSN: 1664-3224

Lipids are organic compounds insoluble in water with a variety of metabolic and non-metabolic functions. They not only represent an efficient energy substrate but can also act as key inflammatory and anti-inflammatory molecules as part of a network of soluble mediators at the interface of metabolism and the immune system. The role of endogenous bioactive lipid mediators has been demonstrated in several inflammatory diseases (rheumatoid arthritis, inflammatory bowel disease, atherosclerosis, cancer). The liver is unique in providing balanced immunotolerance to the exposure of bacterial components from the gut transiting through the portal vein and the lymphatic system. This balance is abruptly deranged in liver failure syndromes such as acute liver failure and acute-on-chronic liver failure. In these syndromes, researchers have recently focused on bioactive lipid mediators by global metabonomic profiling and uncovered the pivotal role of these mediators in the immune dysfunction observed in liver failure syndromes explaining the high occurrence of sepsis and subsequent organ failure. Among endogenous bioactive lipids, the mechanistic actions of three classes (eicosanoids, pro-resolving lipid mediators and lysophospholipids) in the pathophysiological modulation of liver failure syndromes will be the topic of this narrative review. Furthermore, the therapeutic potential of lipid-immune pathways will be described.

Journal article

Flint E, Triantafyllou E, Bernsmeier C, 2022, TAM receptors in the pathophysiology of liver disease, Livers, Vol: 2, Pages: 15-29, ISSN: 2673-4389

TAM receptors (Tyro3, Axl and MerTK) are a family of tyrosine kinase receptors that are expressed in a variety of cell populations, including liver parenchymal and non-parenchymal cells. These receptors are vital for immune homeostasis, as they regulate the innate immune response by suppressing inflammation via toll-like receptor inhibition and by promoting tissue resolution through efferocytosis. However, there is increasing evidence indicating that aberrant TAM receptor signaling may play a role in pathophysiological processes in the context of liver disease. This review will explore the roles of TAM receptors and their ligands in liver homeostasis as well as a variety of disease settings, including acute liver injury, steatosis, fibrosis, cirrhosis-associated immune dysfunction and hepatocellular carcinoma. A better understanding of our current knowledge of TAM receptors in liver disease may identify new opportunities for disease monitoring as well as novel therapeutic targets. Nonetheless, this review also aims to highlight areas where further research on TAM receptor biology in liver disease is required.

Journal article

Cavazza A, Huang X, Triantafyllou E, Ma Y, Antoniades CG, Lee WM, McPhail MJ, Karvellas CJet al., 2021, OSTEOPONTIN, MACROPHAGE MANNOSE RECEPTOR AND SOLUBLE PD-L1 ARE UPREGULATED MACROPHAGE ACTIVATION MARKERS IN PATIENTS WITH ACUTE LIVER FAILURE, Publisher: WILEY, Pages: 164A-165A, ISSN: 0270-9139

Conference paper

Roth S, Flint E, Ghataore L, Patel VC, Singanayagam A, Vincent RP, Triantafyllou E, Ma Y, Bernal W, Auzinger G, Heneghan M, McPhail M, Antoniades C, Christ-Crain M, Taylor DR, Wendon J, Bernsmeier Cet al., 2021, Low baseline but not delta cortisol relates to 28-day transplant-free survival in acute liver failure syndromes, Publisher: E M H SWISS MEDICAL PUBLISHERS LTD, Pages: 25-25, ISSN: 1424-7860

Conference paper

Khamri W, Gudd C, Liu T, Nathwani R, Krasniqi M, Azam S, Barbera T, Trovato FM, Possamai LA, Triantafyllou E, Castro Seoane R, Lebosse F, Singanayagam A, Kumar N, Bernsmeier C, Mukherjee S, McPhail MJW, Weston CJ, Antoniades CG, Thursz MRet al., 2021, Suppressor CD4+ T cells expressing HLA-G are expanded in the peripheral blood from patients with acute decompensation of cirrhosis, Gut, ISSN: 0017-5749

Objective Identifying components of immuneparesis, a hallmark of chronic liver failure, is crucial for our understanding of complications in cirrhosis. Various suppressor CD4+ T cells have been established as potent inhibitors of systemic immune activation. Here, we establish the presence, regulation and mechanism of action of a suppressive CD4+ T cell subset expressing human leucocyte antigen G (HLA-G) in patients with acute decompensation of cirrhosis (AD).Design Flow cytometry was used to determine the proportion and immunophenotype of CD4+HLA-G+ T cells from peripheral blood of 20 healthy controls (HCs) and 98 patients with cirrhosis (28 with stable cirrhosis (SC), 20 with chronic decompensated cirrhosis (CD) and 50 with AD). Transcriptional and functional signatures of cell-sorted CD4+HLA-G+ cells were delineated by NanoString technology and suppression assays, respectively. The role of immunosuppressive cytokine interleukin (IL)-35 in inducing this population was investigated through in vitro blockade experiments. Immunohistochemistry (IHC) and cultures of primary human Kupffer cells (KCs) were performed to assess cellular sources of IL-35. HLA-G-mediated T cell suppression was explored using neutralising antibodies targeting co-inhibitory pathways.Results Patients with AD were distinguished by an expansion of a CD4+HLA-G+CTLA-4+IL-35+ immunosuppressive population associated with disease severity, clinical course of AD, infectious complications and poor outcome. Transcriptomic analyses excluded the possibility that these were thymic-derived regulatory T cells. IHC analyses and in vitro cultures demonstrate that KCs represent a potent source of IL-35 which can induce the observed HLA-G+ phenotype. These exert cytotoxic T lymphocyte antigen-4-mediated impaired responses in T cells paralleled by an HLA-G-driven downregulation of T helper 17-related cytokines.Conclusion We have identified a cytokine-driven peripherally derived suppressive population that may contr

Journal article

Trovato FM, Zia R, Napoli S, Wolfer K, Huang X, Morgan PE, Husbyn H, Elgosbi M, Lucangeli M, Miquel R, Wilson I, Heaton ND, Heneghan MA, Auzinger G, Antoniades CG, Wendon JA, Patel VC, Coen M, Triantafyllou E, McPhail MJet al., 2021, Dysregulation of the Lysophosphatidylcholine/Autotaxin/Lysophosphatidic Acid Axis in Acute‐on‐Chronic Liver Failure Is Associated With Mortality and Systemic Inflammation by Lysophosphatidic Acid–Dependent Monocyte Activation, Hepatology, Vol: 74, Pages: 907-925, ISSN: 0270-9139

Journal article

Gudd CLC, Antoniades CG, Turajlic S, Gore M, Larkin J, Thursz MR, Khamri W, Goldin RD, Woollard KJ, Triantafyllou E, Possamai LAet al., 2021, CCR2+monocytes induce hepatic accumulation and activation of tissue destructive CD8+T cells in a murine model of checkpoint inhibitor-induced hepatitis, Publisher: ELSEVIER, Pages: S224-S225, ISSN: 0168-8278

Conference paper

Roth S, Flint E, Ghataore L, Patel VC, Singanayagam A, Vincent RP, Triantafyllou E, Ma Y, Bernal W, Auzinger G, Heneghan M, McPhail M, Antoniades C, Christ-Crain M, Taylor DR, Wendon J, Bernsmeier Cet al., 2021, Low baseline cortisol but not delta cortisol relates to 28-day transplant-free survival in acute liver failure syndromes, Publisher: ELSEVIER, Pages: S306-S306, ISSN: 0168-8278

Conference paper

Triantafyllou E, Trovato F, Husbyn H, Huang X, Mukherjee S, Bernsmeier C, Goldin RD, Wendon J, Possamai L, Khamri W, Thursz M, McPhail MJWet al., 2021, PD-L1 expression of monocytes relates to severity, infection and mortality in acute-on-chronic liver failure, Publisher: ELSEVIER, Pages: S208-S208, ISSN: 0168-8278

Conference paper

Gudd CLC, Au L, Triantafyllou E, Shum B, Liu T, Nathwani R, Kumar N, Mukherjee S, Dhar A, Woollard KJ, Yone Y, Pinato DJ, Thursz MR, Goldin RD, Gore ME, Larkin J, Khamri W, Antoniades CG, Turajlic S, Possamai LAet al., 2021, Activation and transcriptional profile of monocytes and CD8+ T cells are altered in checkpoint inhibitor-related hepatitis, Journal of Hepatology, Vol: 75, Pages: 177-189, ISSN: 0168-8278

Journal article

Singanayagam A, Triantafyllou E, 2021, Macrophages in Chronic Liver Failure: Diversity, Plasticity and Therapeutic Targeting, Frontiers in Immunology, Vol: 12

<jats:p>Chronic liver injury results in immune-driven progressive fibrosis, with risk of cirrhosis development and impact on morbidity and mortality. Persistent liver cell damage and death causes immune cell activation and inflammation. Patients with advanced cirrhosis additionally experience pathological bacterial translocation, exposure to microbial products and chronic engagement of the immune system. Bacterial infections have a high incidence in cirrhosis, with spontaneous bacterial peritonitis being the most common, while the subsequent systemic inflammation, organ failure and immune dysregulation increase the mortality risk. Tissue-resident and recruited macrophages play a central part in the development of inflammation and fibrosis progression. In the liver, adipose tissue, peritoneum and intestines, diverse macrophage populations exhibit great phenotypic and functional plasticity determined by their ontogeny, epigenetic programming and local microenvironment. These changes can, at different times, promote or ameliorate disease states and therefore represent potential targets for macrophage-directed therapies. In this review, we discuss the evidence for macrophage phenotypic and functional alterations in tissue compartments during the development and progression of chronic liver failure in different aetiologies and highlight the potential of macrophage modulation as a therapeutic strategy for liver disease.</jats:p>

Journal article

Triantafyllou E, Gudd CLC, Mawhin M-A, Husbyn HC, Trovato FM, Siggins MK, OConnor T, Kudo H, Mukherjee SK, Wendon JA, Bernsmeier C, Goldin RD, Botto M, Khamri W, McPhail MJW, Possamai LA, Woollard KJ, Antoniades CG, Thursz MRet al., 2021, PD-1 blockade improves Kupffer cell bacterial clearance in acute liver injury, Journal of Clinical Investigation, Vol: 131, ISSN: 0021-9738

Journal article

Brenig R, Pop OT, Triantafyllou E, Geng A, Singanayagam A, Perez-Shibayama C, Besse L, Cupovic J, Kuenzler P, Boldanova T, Brand S, Semela D, Duong FHT, Weston CJ, Ludewig B, Heim MH, Wendon J, Antoniades CG, Bernsmeier Cet al., 2020, Expression of AXL receptor tyrosine kinase relates to monocyte dysfunction and severity of cirrhosis, Life Science Alliance, Vol: 3, Pages: 1-16, ISSN: 2575-1077

Infectious complications in patients with cirrhosis frequently initiate episodes of decompensation and substantially contribute to the high mortality. Mechanisms of the underlying immuneparesis remain underexplored. TAM receptors (TYRO3/AXL/MERTK) are important inhibitors of innate immune responses. To understand the pathophysiology of immuneparesis in cirrhosis, we detailed TAM receptor expression in relation to monocyte function and disease severity prior to the onset of acute decompensation. TNF-α/IL-6 responses to lipopolysaccharide were attenuated in monocytes from patients with cirrhosis (n = 96) compared with controls (n = 27) and decreased in parallel with disease severity. Concurrently, an AXL-expressing (AXL+) monocyte population expanded. AXL+ cells (CD14+CD16highHLA-DRhigh) were characterised by attenuated TNF-α/IL-6 responses and T cell activation but enhanced efferocytosis and preserved phagocytosis of Escherichia coli. Their expansion correlated with disease severity, complications, infection, and 1-yr mortality. AXL+ monocytes were generated in response to microbial products and efferocytosis in vitro. AXL kinase inhibition and down-regulation reversed attenuated monocyte inflammatory responses in cirrhosis ex vivo. AXL may thus serve as prognostic marker and deserves evaluation as immunotherapeutic target in cirrhosis.

Journal article

Lebosse F, Gudd C, Tunc E, Singanayagam A, Nathwani R, Triantafyllou E, Pop O, Kumar N, Mukherjee S, Zheng Hou T, Quaglia A, Zoulim F, Wendon J, Dhar A, Thursz M, Antoniades C, Khamri Wet al., 2019, CD8+ T cells from patients with cirrhosis display a phenotype that may contribute to cirrhosis-associated immune dysfunction, EBioMedicine, Vol: 49, Pages: 258-268, ISSN: 2352-3964

BackgroundCirrhosis-associated immune dysfunction (CAID) contributes to high sepsis risk in patients with chronic liver disease. Various innate and; to a lesser extent; adaptive immune dysfunctions have been described as contributors to CAID leading to immune-paresis and impaired anti-microbial response in cirrhosis. In this study, we examined the phenotype of CD8+ T cells in chronic liver disease with the aim to evaluate changes that might contribute to impaired immune responses.MethodsSixty patients with cirrhosis were prospectively recruited for this study. CD8+ T cells from peripheral blood, ascites and liver explants were characterized using flow cytometry and immunohistochemistry, respectively. The transcriptional signature of flow-sorted HLA-DR+CD8+ T cells was performed using Nanostring™ technology. HLA-DR+CD8+ T cells interactions with PBMCs and myeloid cells were tested in vitro.FindingsPeripheral CD8+ T cells from cirrhotic patients displayed an altered phenotype characterized by high HLA-DR and TIM-3 surface expression associated with concomitant infections and disease severity, respectively. Paired peritoneal CD8+ T cells expressed more pronounced levels of HLA-DR and PD-1 compared to peripheral CD8+ T cells. HLA-DR+CD8+ T cells were enriched in cirrhotic livers compared to controls. TIM-3, CTLA-4 and PD-1 levels were highly expressed on HLA-DR+CD8+ T cells and co-expression of HLA-DR and PD1 was higher in patients with poor disease outcomes. Genes involved in cytokines production and intracellular signalling pathways were strongly down-regulated in HLA-DR+CD8+ T cells. In comparison to their HLA-DR− counterparts, HLA-DR+CD8+ T cells promoted less proliferation of PBMCs and induced phenotypic and functional dysfunctions in monocytes and neutrophils in vitro.InterpretationIn patients with cirrhosis, CD8+ T cells display a phenotypic, functional and transcriptional profile which may contribute to CAID.FundThis work was supported by Medical Res

Journal article

Ong DJ, Hall A, Triantafyllou E, Pop O, Thursz M, Luong T, Quaglia Aet al., 2019, Expression of Secretory Leukocyte Protease Inhibitor (SLPI) Detected by Immunohistochemistry in Hepatocellular and Cholangiocellular Tumours, 12th Joint Meeting of the British-Division-of-the-International-Academy-of-Pathology and the Pathological-Society-of-Great-Britain-and-Ireland (Leeds Pathology), Publisher: WILEY, Pages: S45-S45, ISSN: 0022-3417

Conference paper

Pop OT, Brenig RG, Triantafyllou E, Kunzler P, Brand S, Wendon JA, Heim MH, Semela D, Antoniades CG, Terracciano LM, Bernsmeier Cet al., 2019, AXL-expressing immune-homeostatic liver macrophages disappear in progressive cirrhosis, Publisher: E M H SWISS MEDICAL PUBLISHERS LTD, Pages: 22S-22S, ISSN: 1424-7860

Conference paper

Singanayagam A, Erminelli D, Triantafyllou E, Bernsmeier C, Tunc E, Krasniqi M, Patel V, Lebosse F, Nathwani R, Atkinson A, Dhar A, Heneghan M, Auzinger G, Khamri W, Adams D, Wendon J, Thursz M, McPhail MJW, Antoniades Het al., 2019, Immunometabolic profiling of ascites from patients with acute-on-chronic liver failure reveals increased MerTK plus immunosuppressive myeloid cells and cell death markers with preferential lipid metabolism compared to cirrhosis without organ failure, International Liver Congress / 54th Annual Meeting of the European-Association-for-the-Study-of-the-Liver (EASL), Publisher: ELSEVIER, Pages: E183-E183, ISSN: 0168-8278

Conference paper

Gudd CLC, Liu T, Triantafyllou E, Pinato DJ, Yone Y, Khamri W, Au L, Woollard KJ, Turajlic S, Goldin RD, Thursz MR, Antoniades CG, Possamai LAet al., 2019, Elevated tissue homing of monocytes and increased cytotoxic activity of CD8+T-cells in immune checkpoint inhibitor-induced hepatitis, International Liver Congress / 54th Annual Meeting of the European-Association-for-the-Study-of-the-Liver (EASL), Publisher: ELSEVIER, Pages: E19-E20, ISSN: 0168-8278

Conference paper

Triantafyllou E, Gudd C, Nathwani R, Trovato F, Possamai L, Morrison R, Bernsmeier C, Patel V, Khamri W, Goldin R, Wendon J, McPhail M, Woollard K, Thursz M, Antoniades Cet al., 2019, PD-1+monocytes and macrophages contribute to impaired microbial clearance following acute liver failure, International Liver Congress / 54th Annual Meeting of the European-Association-for-the-Study-of-the-Liver (EASL), Publisher: ELSEVIER, Pages: E430-E431, ISSN: 0168-8278

Conference paper

McPhail MJW, Trovato F, Triantafyllou E, Zia R, Wolfer K, Huang X, Rabinowich L, Patel VC, Holmes E, Heaton N, Heneghan M, Bernal W, Auzinger G, Coen M, Wendon J, Antoniades Het al., 2019, Autotaxin mediates lipid dysregulation in acute-on-chronic liver failure, promoting persistence of systemic inflammation via lysophosphatidic acid-mediated monocyte activation, International Liver Congress / 54th Annual Meeting of the European-Association-for-the-Study-of-the-Liver (EASL), Publisher: ELSEVIER, Pages: E91-E92, ISSN: 0168-8278

Conference paper

Triantafyllou E, Woollard K, McPhail M, Antoniades C, Possamai Let al., 2018, The role of monocytes and macrophages in acute and acute-on-chronic liver failure, Frontiers in Immunology, Vol: 9, ISSN: 1664-3224

Acute and acute-on-chronic liver failure (ALF and ACLF), though distinct clinical entities, are considered syndromes of innate immune dysfunction. Patients with ALF and ACLF display evidence of a pro-inflammatory state with local liver inflammation, features of systemic inflammatory response syndrome (SIRS) and vascular endothelial dysfunction that drive progression to multi-organ failure. In an apparent paradox, these patients are concurrently immunosuppressed, exhibiting acquired immune defects that render them highly susceptible to infections. This paradigm of tissue injury succeeded by immunosuppression is seen in other inflammatory conditions such as sepsis, which share poor outcomes and infective complications that account for high morbidity and mortality. Monocyte and macrophage dysfunction are central to disease progression of ALF and ACLF. Activation of liver-resident macrophages (Kupffer cells) by pathogen and damage associated molecular patterns leads to the recruitment of innate effector cells to the injured liver. Early monocyte infiltration may contribute to local tissue destruction during the propagation phase and results in secretion of pro-inflammatory cytokines that drive SIRS. In the hepatic microenvironment, recruited monocytes mature into macrophages following local reprogramming so as to promote resolution responses in a drive to maintain tissue integrity. Intra-hepatic events may affect circulating monocytes through spill over of soluble mediators and exposure to apoptotic cell debris during passage through the liver. Hence, peripheral monocytes show numerous acquired defects in acute liver failure syndromes that impair their anti-microbial programmes and contribute to enhanced susceptibility to sepsis. This review will highlight the cellular and molecular mechanisms by which monocytes and macrophages contribute to the pathophysiology of ALF and ACLF, considering both hepatic inflammation and systemic immunosuppression. We identify areas for f

Journal article

Brenig R, Pop OT, Perez C, Duong FHT, Kunzler P, Wieland S, Triantafyllou E, Singanayagam A, Boldanova T, Brand S, Terracciano LM, Wendon JA, Heim MH, Semela D, Antoniades H, Bernsmeier Cet al., 2018, AXL-expressing immunoregulatory monocytes indicate disease severity in patients with advanced cirrhosis, Publisher: E M H SWISS MEDICAL PUBLISHERS LTD, Pages: 10S-10S, ISSN: 1424-7860

Conference paper

Singanayagam A, Nathwani R, Triantafyllou E, Patel VC, Dhar A, McPhail M, Bernsmeier C, Wendon J, Antoniades CGet al., 2018, PLASMA S100A8/A9: A NOVEL MECHANISTIC BIOMARKER IN INNATE IMMUNE ACTIVATION IN ACUTE-ON-CHRONIC LIVER FAILURE, Annual General Meeting of the British-Society-of-Gastroenterology, Publisher: BMJ PUBLISHING GROUP, Pages: A131-A132, ISSN: 0017-5749

Conference paper

Bernsmeier C, Triantafyllou E, Brenig R, Lebosse FJ, Singanayagam A, Patel VC, Pop OT, Khamri W, Nathwani R, Tidswell R, Weston CJ, Adams DH, Thursz MR, Wendon JA, Antoniades CGet al., 2018, CD14<sup>+ </sup>CD15<sup>− </sup>HLA-DR<sup>−</sup> myeloid-derived suppressor cells impair antimicrobial responses in patients with acute-on-chronic liver failure, Gut, Vol: 67, Pages: 1155-1167, ISSN: 0017-5749

<jats:sec><jats:title>Objective</jats:title><jats:p>Immune paresis in patients with acute-on-chronic liver failure (ACLF) accounts for infection susceptibility and increased mortality. Immunosuppressive mononuclear CD14<jats:sup>+</jats:sup>HLA-DR<jats:sup>−</jats:sup> myeloid-derived suppressor cells (M-MDSCs) have recently been identified to quell antimicrobial responses in immune-mediated diseases. We sought to delineate the function and derivation of M-MDSC in patients with ACLF, and explore potential targets to augment antimicrobial responses.</jats:p></jats:sec><jats:sec><jats:title>Design</jats:title><jats:p>Patients with ACLF (n=41) were compared with healthy subjects (n=25) and patients with cirrhosis (n=22) or acute liver failure (n=30). CD14<jats:sup>+</jats:sup>CD15<jats:sup>−</jats:sup>CD11b<jats:sup>+</jats:sup>HLA-DR<jats:sup>−</jats:sup> cells were identified as per definition of M-MDSC and detailed immunophenotypic analyses were performed. Suppression of T cell activation was assessed by mixed lymphocyte reaction. Assessment of innate immune function included cytokine expression in response to Toll-like receptor (TLR-2, TLR-4 and TLR-9) stimulation and phagocytosis assays using flow cytometry and live cell imaging-based techniques.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>Circulating CD14<jats:sup>+</jats:sup>CD15<jats:sup>−</jats:sup>CD11b<jats:sup>+</jats:sup>HLA-DR<jats:sup>−</jats:sup> M-MDSCs were markedly expanded in patients with ACLF (55% of CD14+ cells). M-MDSC displayed immunosuppressive properties, significantly decreasing T cell proliferation (p=0.01), producing less tumour necrosis factor-alpha/interleukin-6 in response to TLR stimulation (all p&lt;0.01), and redu

Journal article

Kumar N, Khamri W, Sadiq F, Triantafyllou E, Lebosse F, Sujit M, Dhar A, Thursz M, Antoniades H, Khakoo Set al., 2018, Circulating NK cells in cirrhosis are hypofunctional, with an expanded inhibitory liver-homing CD56dimCD16+/- NK cell population, International Liver Congress (ILC), Publisher: ELSEVIER SCIENCE BV, Pages: S611-S611, ISSN: 0168-8278

Conference paper

Brenig R, Perez C, Duong FHT, Kuenzler P, Besse L, Wieland S, Pop O, Triantafyllou E, Boldanova T, Brand S, Terraciano L, Wendon J, Heim M, Semela D, Antoniades H, Bernsmeier Cet al., 2018, AXL-expressing monocytes indicate immuneparesis and disease severity in patients with cirrhosis, International Liver Congress (ILC), Publisher: ELSEVIER SCIENCE BV, Pages: S613-S613, ISSN: 0168-8278

Conference paper

Triantafyllou E, Pop O, Possamai L, Wilhelm A, Liaskou E, Petts G, Singanayagam A, Bernsmeier C, Khamri W, Patel V, Stamataki Z, Curbishley S, Ma Y, Woollard K, Quaglia A, Wendon J, Thursz M, Adams D, Weston C, Antoniades Cet al., 2017, MERTK EXPRESSING HEPATIC MACROPHAGES PROMOTE THE RESOLUTION OF INFLAMMATION IN ACUTE LIVER FAILURE, Annual General Meeting of the British-Society-of-Gastroenterology (BSG), Publisher: BMJ PUBLISHING GROUP, Pages: A48-A49, ISSN: 0017-5749

Conference paper

Khamri W, Abeles RD, Hou TZ, Anderson AE, El-Masry A, Triantafyllou E, Bernsmeier C, Larsen FS, Singanayagam A, Kudo N, Possamai LA, Lebosse F, Auzinger G, Bernal W, Willars C, Weston CJ, Lombardi G, Wendon J, Thursz M, Antoniades CGet al., 2017, Increased Expression of Cytotoxic T-Lymphocyte−Associated Protein 4 by T Cells, Induced by B7 in Sera, Reduces Adaptive Immunity in Patients With Acute Liver Failure, Gastroenterology, Vol: 153, Pages: 263-276.e8, ISSN: 0016-5085

Journal article

Triantafyllou E, Pop O, Possamai L, Wilhelm A, Liaskou E, Singanayagam A, Bernsmeier C, Khamri W, Petts G, Dargue R, Davies S, Tickle J, Yuksel M, Patel V, Abeles R, Stamataki Z, Curbishley S, Ma Y, Wilson I, Coen M, Woollard K, Quaglia A, Wendon J, Thursz M, Adams D, Weston C, Antoniades Cet al., 2017, MerTK expressing hepatic macrophages promote the resolution of inflammation in acute liver failure, Gut, Vol: 67, Pages: 333-347, ISSN: 1468-3288

Objective Acute liver failure (ALF) is characterised by overwhelming hepatocyte death and liver inflammation with massive infiltration of myeloid cells in necrotic areas. The mechanisms underlying resolution of acute hepatic inflammation are largely unknown. Here, we aimed to investigate the impact of Mer tyrosine kinase (MerTK) during ALF and also examine how the microenvironmental mediator, secretory leucocyte protease inhibitor (SLPI), governs this response.Design Flow cytometry, immunohistochemistry, confocal imaging and gene expression analyses determined the phenotype, functional/transcriptomic profile and tissue topography of MerTK+ monocytes/macrophages in ALF, healthy and disease controls. The temporal evolution of macrophage MerTK expression and its impact on resolution was examined in APAP-induced acute liver injury using wild-type (WT) and Mer-deficient (Mer−/−) mice. SLPI effects on hepatic myeloid cells were determined in vitro and in vivo using APAP-treated WT mice.Results We demonstrate a significant expansion of resolution-like MerTK+HLA-DRhigh cells in circulatory and tissue compartments of patients with ALF. Compared with WT mice which show an increase of MerTK+MHCIIhigh macrophages during the resolution phase in ALF, APAP-treated Mer−/− mice exhibit persistent liver injury and inflammation, characterised by a decreased proportion of resident Kupffer cells and increased number of neutrophils. Both in vitro and in APAP-treated mice, SLPI reprogrammes myeloid cells towards resolution responses through induction of a MerTK+HLA-DRhigh phenotype which promotes neutrophil apoptosis and their subsequent clearance.Conclusions We identify a hepatoprotective, MerTK+, macrophage phenotype that evolves during the resolution phase following ALF and represents a novel immunotherapeutic target to promote resolution responses following acute liver injury.

Journal article

Mukherjee S, Pop O, Triantafyllou E, Khamri W, Curbishley S, Thursz M, Adams D, Antoniades Cet al., 2017, Role of expression of the tumour-associated macrophage receptor, MERTK, in hepatocellular carcinoma, Spring Meeting on Clinician Scientists in Training, Publisher: ELSEVIER SCIENCE INC, Pages: 72-72, ISSN: 0140-6736

Conference paper

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