Dr Elizabeth Want

Want EJ, Wilson ID, Gika H, Theodoridis G, Plumb RS, Shockcor J, Holmes E, Nicholson JKet al., 2010, Global metabolic profiling procedures for urine using UPLC-MS, NATURE PROTOCOLS, Vol: 5, Pages: 1005-1018, ISSN: 1754-2189

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• Citations: 760

Coen M, Want EJ, Clayton TA, Rohde CM, Hong YS, Keun HC, Cantor GC, Metz AL, Robertson DG, Reily MD, Holmes E, Lindon JC, Nicholson JKet al., 2009, Mechanistic aspects and novel biomarkers of responder and non-responder phenotypes in galactosamine-induced hepatitis, Journal of Proteome Research, Vol: 8, Pages: 5175-5187, ISSN: 1535-3907

The amino sugar galactosamine (galN) induces alterations in the hepatic uridine nucleotide pool and has been widely used as a model of human viral hepatitis. Histopathological and clinical chemistry analyses of a cohort of rats following administration of galN revealed extreme interindividual variability in the extent of the toxic response which enabled classification of ‘responder’ and ‘non-responder’ phenotypes. An integrative metabolic profiling approach was applied to characterize biomarkers of exposure to galN in urine, serum, feces and liver from responders and non-responders. The presence of N-acetylglucosamine and galN in the urine correlated with the occurrence and extent of toxic response. Conversely, the novel identification of galN-pyrazines in the feces of non-responders and their virtual absence in the feces of responders suggests an alternative means of distribution and metabolism of galN in non-responders. The absence of the UDP-hexosamines in the liver of non-responders further supports differential metabolism of galN and suggests an ability of non- responders to avoid UDP-glucose depletion. An observed disturbance of gut microbial derived metabolites in the urine and feces of non-responders may suggest a role of the microflora in reducing the effective dose of galN. This systems level metabonomic approach has provided new mechanistic insights into differential response to galN and is widely applicable to the study of interindividual variation in metabolism for any xenobiotic intervention.

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Stuerzenbaum SR, Zeitoun-Ghandour SM, Hodson M, Charnock JM, Bundy JG, Want EJ, Hughes S, Swain SCet al., 2009, Characterization of <i>Caenorhabditis elegans</i> metallothioneins: Genomics, metabolomics and XAFS, 26th Congress of the European-Society-of-Comparative-Biochemistry-and-Physiology, Publisher: ELSEVIER SCIENCE INC, Pages: S16-S16, ISSN: 1095-6433

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Want E, 2009, Challenges in applying chemometrics to LC-MS-based global metabolite profile data, BIOANALYSIS, Vol: 1, Pages: 805-819, ISSN: 1757-6180

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• Citations: 16

Hughes SL, Bundy JG, Want EJ, Kille P, Sturzenbaum SRet al., 2009, The Metabolomic Responses of <i>Caenorhabditis elegans</i> to Cadmium Are Largely Independent of Metallothionein Status, but Dominated by Changes in Cystathionine and Phytochelatins, JOURNAL OF PROTEOME RESEARCH, Vol: 8, Pages: 3512-3519, ISSN: 1535-3893

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• Citations: 95

Smith LM, Maher AD, Want EJ, Elliott P, Stamler J, Hawkes GE, Holmes E, Lindon JC, Nicholson JKet al., 2009, Large-Scale Human Metabolic Phenotyping and Molecular Epidemiological Studies-via <SUP>1</SUP>H NMR Spectroscopy of Urine: Investigation of Borate Preservation, ANALYTICAL CHEMISTRY, Vol: 81, Pages: 4847-4856, ISSN: 0003-2700

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• Citations: 25

Maher AD, Patki P, Lindon JC, Want EJ, Holmes E, Craggs M, Nicholson JKet al., 2008, Seminal Oligouridinosis: Low Uridine Secretion as a Biomarker for Infertility in Spinal Neurotrauma, CLINICAL CHEMISTRY, Vol: 54, Pages: 2063-2066, ISSN: 0009-9147

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• Citations: 17

Nordström A, Want E, Northen T, Lehtiö J, Siuzdak Get al., 2008, Multiple ionization mass spectrometry strategy used to reveal the complexity of metabolomics., Anal Chem, Vol: 80, Pages: 421-429, ISSN: 0003-2700

A multiple ionization mass spectrometry strategy is presented based on the analysis of human serum extracts. Chromatographic separation was interfaced inline with the atmospheric pressure ionization techniques electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) in both positive (+) and negative (-) ionization modes. Furthermore, surface-based matrix-assisted laser desorption/ionization (MALDI) and desorption ionization on silicon (DIOS) mass spectrometry were also integrated with the separation through fraction collection and offline mass spectrometry. Processing of raw data using the XCMS software resulted in time-aligned ion features, which are defined as a unique m/z at a unique retention time. The ion feature lists obtained through LC-MS with ESI and APCI interfaces in both +/- ionization modes were compared, and unique ion tables were generated. Nonredundant, unique ion features, were defined as mass numbers for which no mass numbers corresponding to [M + H](+), [M - H](-), or [M + Na](+) were observed in the other ionization methods at the same retention time. Analysis of the extracted serum using ESI for both (+) and (-) ions resulted in >90% additional unique ions being detected in the (-) ESI mode. Complementing the ESI analysis with APCI resulted in an additional approximately 20% increase in unique ions. Finally, ESI/APCI ionization was combined with fraction collection and offline-MALDI and DIOS mass spectrometry. The parts of the total ion current chromatograms in the LC-MS acquired data corresponding to collected fractions were summed, and m/z lists were compiled and compared to the m/z lists obtained from the DIOS/MALDI spectra. It was observed that, for each fraction, DIOS accounted for approximately 50% of the unique ions detected. These results suggest that true global metabolomics will require multiple ionization technologies to address the inherent metabolite diversity and therefore the complexity in and of metabo

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Nordstrom A, Want E, Northen T, Lehtioe J, Siuzdak Get al., 2008, Multiple ionization mass spectrometry strategy used to reveal the complexity of metabolomics, ANALYTICAL CHEMISTRY, Vol: 80, Pages: 421-429, ISSN: 0003-2700

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• Citations: 145

O'Maille G, Go EP, Hoang L, Want EJ, Smith C, O'Maille P, Nordstrom A, Morita H, Qin C, Uritboonthai W, Apon J, Moore R, Garrett J, Siuzdak Get al., 2008, Metabolomics relative quantitation with mass spectrometry using chemical derivatization and isotope labeling, SPECTROSCOPY-AN INTERNATIONAL JOURNAL, Vol: 22, Pages: 327-343, ISSN: 0712-4813

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• Citations: 16

Want EJ, Nordstrom A, Morita H, Siuzdak Get al., 2007, From exogenous to endogenous:: The inevitable imprint of mass spectrometry in metabolomics, JOURNAL OF PROTEOME RESEARCH, Vol: 6, Pages: 459-468, ISSN: 1535-3893

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• Citations: 231

Shen Z, Want EJ, Chen W, Keating W, Nussbaumer W, Moore R, Gentle TM, Siuzdak Get al., 2006, Sepsis plasma protein profiling with immunodepletion, three-dimensional liquid chromatography tandem mass spectrometry, and spectrum counting, JOURNAL OF PROTEOME RESEARCH, Vol: 5, Pages: 3154-3160, ISSN: 1535-3893

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• Citations: 55

Pendyala G, Want EJ, Webb W, Siuzdak G, Fox Het al., 2006, Biomarkers for NeuroAIDS: The Widening Scope of Metabolomics, Journal of NeuroImmune Pharmacology, Vol: online first

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Want EJ, Smith CA, Qin C, Van Horne KC, Siuzdak Get al., 2006, Phospholipid capture combined with non-linear chromatographic correction for improved serum metabolite profiling, Metabolomics, Vol: 2 (3), Pages: 145-154

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Want EJ, O'Maille G, Smith CA, Brandon TR, Uritboonthai W, Qin C, Trauger SA, Siuzdak Get al., 2006, Solvent-dependent metabolite distribution, clustering, and protein extraction for serum profiling with mass spectrometry, ANALYTICAL CHEMISTRY, Vol: 78, Pages: 743-752, ISSN: 0003-2700

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• Citations: 352

Smith CA, Want EJ, O'Maille G, Abagyan R, Siuzdak Get al., 2006, XCMS: Processing mass spectrometry data for metabolite profiling using Nonlinear peak alignment, matching, and identification, ANALYTICAL CHEMISTRY, Vol: 78, Pages: 779-787, ISSN: 0003-2700

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• Citations: 3344

Smith CA, O'Maille G, Want EJ, Qin C, Trauger SA, Brandon TR, Custodio DE, Abagyan R, Siuzdak Get al., 2005, METLIN - A metabolite mass spectral database, THERAPEUTIC DRUG MONITORING, Vol: 27, Pages: 747-751, ISSN: 0163-4356

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• Citations: 1650

Want EJ, Cravatt BF, Siuzdak G, 2005, The expanding role of mass spectrometry in metabolite profiling and characterization, CHEMBIOCHEM, Vol: 6, Pages: 1941-1951, ISSN: 1439-4227

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• Citations: 187

Want EJ, Tong G, Shen ZX, Abagyan R, Smith CAet al., 2005, Metabolite profiling in disease., 229th National Meeting of the American-Chemical-Society (ACS), Publisher: AMER CHEMICAL SOC, Pages: U148-U148, ISSN: 0065-7727

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Saghatelian A, Trauger SA, Want EJ, Hawkins EG, Siuzdak G, Cravatt BFet al., 2004, Assignment of endogenous substrates to enzymes by global metabolite profiling, BIOCHEMISTRY, Vol: 43, Pages: 14332-14339, ISSN: 0006-2960

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• Citations: 269

Barker G, Want EJ, Boydston J, Siuzdak Get al., 2003, Mass Spectrometry, Science of Synthesis, Editors: Goodman, Diego, Felix, Mahwah, Moroder, Martinsried, Toniolo, Padova, Italy, Publisher: Houben-Weyl Thieme Chemistry, Pages: 680-695

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Want EJ, Compton BJ, Hollenbeck T, Siuzdak Get al., 2003, The Application of Mass Spectrometry in Pharmacokinetics, Spectroscopy, Vol: 17, Pages: 681-691

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Want EJ, Greig M, Compton BJ, Bolanos B, Siuzdak Get al., 2003, Mass Spectrometry in High Throughput Analysis, Spectroscopy, Vol: 17, Pages: 663-680

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Adachi J, Kudo R, Ueno Y, Hunter R, Rajendram R, Want E, Preedy VRet al., 2001, Heart 7-hydroperoxycholesterol and oxysterols are elevated in chronically ethanol-fed rats, JOURNAL OF NUTRITION, Vol: 131, Pages: 2916-2920, ISSN: 0022-3166

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• Citations: 35

Rider JR, Want EJ, Winter MA, Turton JR, Pamphilon DH, Nobes Pet al., 2000, Differential leucocyte subpopulation analysis of leucodepleted red cell products, TRANSFUSION MEDICINE, Vol: 10, Pages: 49-58, ISSN: 0958-7578

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• Citations: 15

Claus SP, Ellero SL, Berger B, Krause L, Bruttin A, Molina J, Paris A, Want EJ, de Waziers I, Cloarec O, Richards SE, Wang Y, Dumas M-E, Ross A, Rezzi S, Kochhar S, Van Bladeren P, Lindon JC, Holmes E, Nicholson JKet al., Colonization-Induced Host-Gut Microbial Metabolic Interaction, MBIO, Vol: 2, ISSN: 2150-7511

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• Citations: 307