Imperial College London
Alternate

Dr Elizabeth Want

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Senior Lecturer
 
 
 
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Contact

 

+44 (0)20 7594 3023e.want

 
 
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Location

 

E315CBurlington DanesHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Ahmetaj-Shala:2018:10.1038/s41598-018-31055-8,
author = {Ahmetaj-Shala, B and Olanipekun, M and Tesfai, A and MacCallum, N and Kirkby, N and Qunilan, G and Shih, C-C and Kawai, R and Mumby, S and Paul-Clark, M and Want, E and Mitchell, JA},
doi = {10.1038/s41598-018-31055-8},
journal = {Scientific Reports},
title = {Development of a novel UPLC-MS/MS-based platform to quantify amines, amino acids and methylarginines for applications in human disease phenotyping},
url = {http://dx.doi.org/10.1038/s41598-018-31055-8},
volume = {8},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Amine quantification is an important strategy in patient stratification and personalised medicine. This is because amines, including amino acids and methylarginines impact on many homeostatic processes. One important pathway regulated by amine levels is nitric oxide synthase (NOS). NOS is regulated by levels of (i) the substrate, arginine, (ii) amino acids which cycle with arginine and (iii) methylarginine inhibitors of NOS. However, biomarker research in this area is hindered by the lack of a unified analytical platform. Thus, the development of a common metabolomics platform, where a wide range of amino acids and methylarginines can be measured constitutes an important unmet need. Here we report a novel high-throughput ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) platform where ≈40 amine analytes, including arginine and methylarginines can be detected and quantified on a molar basis, in a single sample of human plasma. To validate the platform and to generate biomarkers, human plasma from a well-defined cohort of patients before and after coronary artery bypass surgery, who developed systemic inflammatory response syndrome (SIRS), were analysed. Bypass surgery with SIRS significantly altered 26 amine analytes, including arginine and ADMA. Consequently, pathway analysis revealed significant changes in a range of pathways including those associated with NOS.
AU  - Ahmetaj-Shala,B
AU  - Olanipekun,M
AU  - Tesfai,A
AU  - MacCallum,N
AU  - Kirkby,N
AU  - Qunilan,G
AU  - Shih,C-C
AU  - Kawai,R
AU  - Mumby,S
AU  - Paul-Clark,M
AU  - Want,E
AU  - Mitchell,JA
DO  - 10.1038/s41598-018-31055-8
PY  - 2018///
SN  - 2045-2322
TI  - Development of a novel UPLC-MS/MS-based platform to quantify amines, amino acids and methylarginines for applications in human disease phenotyping
T2  - Scientific Reports
UR  - http://dx.doi.org/10.1038/s41598-018-31055-8
UR  - http://hdl.handle.net/10044/1/62685
VL  - 8
ER  -
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