Imperial College London
Alternate

ProfessorElaineHolmes

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Professor of Chemical Biology
 
 
 
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Contact

 

+44 (0)20 7594 3220elaine.holmes

 
 
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Location

 

661Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Alsaleh:2019:10.1016/j.jceh.2019.04.053,
author = {Alsaleh, M and Barbera, TA and Andrews, RH and Sithithaworn, P and Khuntikeo, N and Loilome, W and Yongvanit, P and Cox, IJ and Syms, RRA and Holmes, E and Taylor-Robinson, SD},
doi = {10.1016/j.jceh.2019.04.053},
journal = {Journal of Clinical and Experimental Hepatology},
pages = {597--606},
title = {Mass spectrometry: A guide for the clinician},
url = {http://dx.doi.org/10.1016/j.jceh.2019.04.053},
volume = {9},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Metabolic profiling, metabonomics and metabolomics are terms coined in the late 1990s as they emerged as the newest ‘omics’ technology at the time. This line of research enquiry uses spectroscopic analytical platforms, which are mainly nuclear magnetic resonance spectroscopy and mass spectrometry (MS), to acquire a snapshot of metabolites, the end products of a complex biological system. Metabolic profiling enables the detection, quantification and characterisation of metabolites in biofluids, cells and tissues. The source of these compounds can be of endogenous, microbial or exogenous origin, such as dietary or xenobiotic. This results in generating extensive, multivariate spectroscopic data that require specific statistical manipulation, typically performed using chemometric and pattern recognition techniques to reduce its dimensions, facilitate its biological interpretation and allow sample classification and biomarker discovery. Consequently, it is possible to study the dynamic metabolic changes in response to disease, intervention or environmental conditions. In this review, we describe the fundamentals of MS so that clinicians can be literate in the field and are able to interrogate the right scientific questions.
AU  - Alsaleh,M
AU  - Barbera,TA
AU  - Andrews,RH
AU  - Sithithaworn,P
AU  - Khuntikeo,N
AU  - Loilome,W
AU  - Yongvanit,P
AU  - Cox,IJ
AU  - Syms,RRA
AU  - Holmes,E
AU  - Taylor-Robinson,SD
DO  - 10.1016/j.jceh.2019.04.053
EP  - 606
PY  - 2019///
SN  - 0973-6883
SP  - 597
TI  - Mass spectrometry: A guide for the clinician
T2  - Journal of Clinical and Experimental Hepatology
UR  - http://dx.doi.org/10.1016/j.jceh.2019.04.053
UR  - https://www.sciencedirect.com/science/article/pii/S0973688319301276?via%3Dihub
UR  - http://hdl.handle.net/10044/1/72085
VL  - 9
ER  -
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