Imperial College London
Alternate

ProfessorElaineHolmes

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Professor of Chemical Biology
 
 
 
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Contact

 

+44 (0)20 7594 3220elaine.holmes

 
 
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Location

 

661Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Brignardello:2022:ajcn/nqab211,
author = {Brignardello, J and Fountana, S and Posma, JM and Chambers, ES and Nicholson, JK and Wist, J and Frost, G and Garcia-Perez, I and Holmes, E},
doi = {ajcn/nqab211},
journal = {The American Journal of Clinical Nutrition},
pages = {1368--1378},
title = {Characterization of diet-dependent temporal changes in circulating short-chain fatty acid concentrations: a randomized crossover dietary trial},
url = {http://dx.doi.org/10.1093/ajcn/nqab211},
volume = {116},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Background: Production of Short-chain fatty acids (SCFAs) from food is a complex and dynamic saccharolytic fermentation process mediated by both human and gut microbial factors. SCFA production and knowledge of the relationship between SCFA profiles and dietary patterns is lacking. Objective: Temporal changes in SCFA levels in response to two contrasting diets were investigated using a novel GC-MS method.Design: Samples were obtained from a randomized, controlled, crossover trial designed to characterize the metabolic response to four diets. Participants (n=19) undertook these diets during an inpatient stay (of 72-h). Serum samples were collected 2-h after breakfast (AB), lunch (AL) and dinner (AD) on day 3 and a fasting sample (FA) was obtained on day 4. 24-h urine samples were collected on day 3. In this sub-study, samples from the two extreme diets representing a diet with high adherence to WHO healthy eating recommendations and a typical Western diet were analyzed using a bespoke GC-MS method developed to detect and quantify 10 SCFAs and precursors in serum and urine samples. Results: Considerable inter-individual variation in serum SCFA concentrations was observed across all time points and temporal fluctuations were observed for both diets. Although the sample collection timing exerted a greater magnitude of effect on circulating SCFA concentrations, the unhealthy diet was associated with a lower concentration of acetic acid (FA: coefficient=-17.0; standard error (SE)=5.8; p-trend=0.00615), 2-methylbutyric acid (AL: coefficient=-0.1; SE=0.028; p-trend=4.13x10-4 and AD: coefficient =-0.1; SE:=0.028; p-trend=2.28x10-3) and 2-hydroxybutyric acid (FA: coefficient=-15.8; standard error=5.11; p-trend: 4.09x10-3). In contrast lactic acid was significantly higher in the unhealthy diet (AL: coefficient=750.2; standard error=315.2; p-trend=0.024 and AD: coefficient=1219.3; standard error=322.6; p-trend: 8.28x10-4). Conclusion: The GC-MS method allowed robust mapping of
AU  - Brignardello,J
AU  - Fountana,S
AU  - Posma,JM
AU  - Chambers,ES
AU  - Nicholson,JK
AU  - Wist,J
AU  - Frost,G
AU  - Garcia-Perez,I
AU  - Holmes,E
DO  - ajcn/nqab211
EP  - 1378
PY  - 2022///
SN  - 0002-9165
SP  - 1368
TI  - Characterization of diet-dependent temporal changes in circulating short-chain fatty acid concentrations: a randomized crossover dietary trial
T2  - The American Journal of Clinical Nutrition
UR  - http://dx.doi.org/10.1093/ajcn/nqab211
UR  - https://academic.oup.com/ajcn/advance-article/doi/10.1093/ajcn/nqab211/6711581?login=true
UR  - http://hdl.handle.net/10044/1/89647
VL  - 116
ER  -
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