Imperial College London
Alternate

ProfessorElaineHolmes

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Professor of Chemical Biology
 
 
 
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Contact

 

+44 (0)20 7594 3220elaine.holmes

 
 
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Location

 

661Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Nitschke:2022:10.1021/acs.analchem.1c04576,
author = {Nitschke, P and Lodge, S and Kimhofer, T and Masuda, R and Bong, S-H and Hall, D and Schäfer, H and Spraul, M and Pompe, N and Diercks, T and Bernardo-Seisdedos, G and Mato, JM and Millet, O and Susic, D and Henry, A and El-Omar, EM and Holmes, E and Lindon, JC and Nicholson, JK and Wist, J},
doi = {10.1021/acs.analchem.1c04576},
journal = {Analytical Chemistry},
pages = {1333--1341},
title = {J-edited dIffusional proton nuclear magnetic resonance spectroscopic measurement of glycoprotein and supramolecular phospholipid biomarkers of inflammation in human serum.},
url = {http://dx.doi.org/10.1021/acs.analchem.1c04576},
volume = {94},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Proton nuclear magnetic resonance (NMR) N-acetyl signals (Glyc) from glycoproteins and supramolecular phospholipids composite peak (SPC) from phospholipid quaternary nitrogen methyls in subcompartments of lipoprotein particles) can give important systemic metabolic information, but their absolute quantification is compromised by overlap with interfering resonances from lipoprotein lipids themselves. We present a J-Edited DIffusional (JEDI) proton NMR spectroscopic approach to selectively augment signals from the inflammatory marker peaks Glyc and SPCs in blood serum NMR spectra, which enables direct integration of peaks associated with molecules found in specific compartments. We explore a range of pulse sequences that allow editing based on peak J-modulation, translational diffusion, and T2 relaxation time and validate them for untreated blood serum samples from SARS-CoV-2 infected patients (n = 116) as well as samples from healthy controls and pregnant women with physiological inflammation and hyperlipidemia (n = 631). The data show that JEDI is an improved approach to selectively investigate inflammatory signals in serum and may have widespread diagnostic applicability to disease states associated with systemic inflammation.
AU  - Nitschke,P
AU  - Lodge,S
AU  - Kimhofer,T
AU  - Masuda,R
AU  - Bong,S-H
AU  - Hall,D
AU  - Schäfer,H
AU  - Spraul,M
AU  - Pompe,N
AU  - Diercks,T
AU  - Bernardo-Seisdedos,G
AU  - Mato,JM
AU  - Millet,O
AU  - Susic,D
AU  - Henry,A
AU  - El-Omar,EM
AU  - Holmes,E
AU  - Lindon,JC
AU  - Nicholson,JK
AU  - Wist,J
DO  - 10.1021/acs.analchem.1c04576
EP  - 1341
PY  - 2022///
SN  - 0003-2700
SP  - 1333
TI  - J-edited dIffusional proton nuclear magnetic resonance spectroscopic measurement of glycoprotein and supramolecular phospholipid biomarkers of inflammation in human serum.
T2  - Analytical Chemistry
UR  - http://dx.doi.org/10.1021/acs.analchem.1c04576
UR  - https://www.ncbi.nlm.nih.gov/pubmed/34985268
UR  - https://pubs.acs.org/doi/10.1021/acs.analchem.1c04576
UR  - http://hdl.handle.net/10044/1/93794
VL  - 94
ER  -
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