Imperial College London
Alternate

ProfessorElaineHolmes

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Professor of Chemical Biology
 
 
 
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Contact

 

+44 (0)20 7594 3220elaine.holmes

 
 
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Location

 

661Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Masuda:2022:10.1021/acs.analchem.1c05389,
author = {Masuda, R and Lodge, S and Whiley, L and Gray, N and Lawler, N and Nitschke, P and Bong, S-H and Kimhofer, T and Loo, RL and Boughton, B and Zeng, AX and Hall, D and Schaefer, H and Spraul, M and Dwivedi, G and Yeap, BB and Diercks, T and Bernardo-Seisdedos, G and Mato, JM and Lindon, JC and Holmes, E and Millet, O and Wist, J and Nicholson, JK},
doi = {10.1021/acs.analchem.1c05389},
journal = {Anal Chem},
title = {Exploration of Human Serum Lipoprotein Supramolecular Phospholipids Using Statistical Heterospectroscopy in n-Dimensions (SHY-n): Identification of Potential Cardiovascular Risk Biomarkers Related to SARS-CoV-2 Infection.},
url = {http://dx.doi.org/10.1021/acs.analchem.1c05389},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - SARS-CoV-2 infection causes a significant reduction in lipoprotein-bound serum phospholipids give rise to supramolecular phospholipid composite (SPC) signals observed in diffusion and relaxation edited 1H NMR spectra. To characterize the chemical structural components and compartmental location of SPC and to understand further its possible diagnostic properties, we applied a Statistical HeterospectroscopY in n-dimensions (SHY-n) approach. This involved statistically linking a series of orthogonal measurements made on the same samples, using independent analytical techniques and instruments, to identify the major individual phospholipid components giving rise to the SPC signals. Thus, an integrated model for SARS-CoV-2 positive and control adults is presented that relates three identified diagnostic subregions of the SPC signal envelope (SPC1, SPC2, and SPC3) generated using diffusion and relaxation edited (DIRE) NMR spectroscopy to lipoprotein and lipid measurements obtained by in vitro diagnostic NMR spectroscopy and ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). The SPC signals were then correlated sequentially with (a) total phospholipids in lipoprotein subfractions; (b) apolipoproteins B100, A1, and A2 in different lipoproteins and subcompartments; and (c) MS-measured total serum phosphatidylcholines present in the NMR detection range (i.e., PCs: 16.0,18.2; 18.0,18.1; 18.2,18.2; 16.0,18.1; 16.0,20.4; 18.0,18.2; 18.1,18.2), lysophosphatidylcholines (LPCs: 16.0 and 18.2), and sphingomyelin (SM 22.1). The SPC3/SPC2 ratio correlated strongly (r = 0.86) with the apolipoprotein B100/A1 ratio, a well-established marker of cardiovascular disease risk that is markedly elevated during acute SARS-CoV-2 infection. These data indicate the considerable potential of using a serum SPC measurement as a metric of cardiovascular risk based on a single NMR experiment. This is of specific interest in relation to understanding the potential for in
AU  - Masuda,R
AU  - Lodge,S
AU  - Whiley,L
AU  - Gray,N
AU  - Lawler,N
AU  - Nitschke,P
AU  - Bong,S-H
AU  - Kimhofer,T
AU  - Loo,RL
AU  - Boughton,B
AU  - Zeng,AX
AU  - Hall,D
AU  - Schaefer,H
AU  - Spraul,M
AU  - Dwivedi,G
AU  - Yeap,BB
AU  - Diercks,T
AU  - Bernardo-Seisdedos,G
AU  - Mato,JM
AU  - Lindon,JC
AU  - Holmes,E
AU  - Millet,O
AU  - Wist,J
AU  - Nicholson,JK
DO  - 10.1021/acs.analchem.1c05389
PY  - 2022///
TI  - Exploration of Human Serum Lipoprotein Supramolecular Phospholipids Using Statistical Heterospectroscopy in n-Dimensions (SHY-n): Identification of Potential Cardiovascular Risk Biomarkers Related to SARS-CoV-2 Infection.
T2  - Anal Chem
UR  - http://dx.doi.org/10.1021/acs.analchem.1c05389
UR  - https://www.ncbi.nlm.nih.gov/pubmed/35230805
ER  -
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