Imperial College London
Alternate

ProfessorElaineHolmes

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Professor of Chemical Biology
 
 
 
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Contact

 

+44 (0)20 7594 3220elaine.holmes

 
 
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Location

 

661Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Nitschke:2022:10.1039/d2an01097f,
author = {Nitschke, P and Lodge, S and Hall, D and Schaefer, H and Spraul, M and Embade, N and Millet, O and Holmes, E and Wist, J and Nicholson, JK},
doi = {10.1039/d2an01097f},
journal = {The Analyst},
pages = {4213--4221},
title = {Direct low field J-edited diffusional proton NMR spectroscopic measurement of COVID-19 inflammatory biomarkers in human serum.},
url = {http://dx.doi.org/10.1039/d2an01097f},
volume = {147},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - A JEDI NMR pulse experiment incorporating relaxational, diffusional and J-modulation peak editing has been implemented for a low field (80 MHz proton resonance frequency) spectrometer system to measure quantitatively two recently discovered plasma markers of SARS-CoV-2 infection and general inflammation. JEDI spectra capture a unique signature of two biomarker signals from acetylated glycoproteins (Glyc) and the supramolecular phospholipid composite (SPC) signals that are relatively enhanced by the combination of relaxation, diffusion and J-editing properties of the JEDI experiment that strongly attenuate contributions from the other molecular species in plasma. The SPC/Glyc ratio data were essentially identical in the 600 MHz and 80 MHz spectra obtained (R2 = 0.97) and showed significantly different ratios for control (n = 28) versus SARS-CoV-2 positive patients (n = 29) (p = 5.2 × 10-8 and 3.7 × 10-8 respectively). Simplification of the sample preparation allows for data acquisition in a similar time frame to high field machines (∼4 min) and a high-throughput version with 1 min experiment time could be feasible. These data show that these newly discovered inflammatory biomarkers can be measured effectively on low field NMR instruments that do not not require housing in a complex laboratory environment, thus lowering the barrier to clinical translation of this diagnostic technology.
AU  - Nitschke,P
AU  - Lodge,S
AU  - Hall,D
AU  - Schaefer,H
AU  - Spraul,M
AU  - Embade,N
AU  - Millet,O
AU  - Holmes,E
AU  - Wist,J
AU  - Nicholson,JK
DO  - 10.1039/d2an01097f
EP  - 4221
PY  - 2022///
SN  - 0003-2654
SP  - 4213
TI  - Direct low field J-edited diffusional proton NMR spectroscopic measurement of COVID-19 inflammatory biomarkers in human serum.
T2  - The Analyst
UR  - http://dx.doi.org/10.1039/d2an01097f
UR  - https://www.ncbi.nlm.nih.gov/pubmed/35994017
UR  - https://pubs.rsc.org/en/content/articlelanding/2022/AN/D2AN01097F
UR  - http://hdl.handle.net/10044/1/99342
VL  - 147
ER  -
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