Imperial College London
Alternate

ProfessorElaineHolmes

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Professor of Chemical Biology
 
 
 
//

Contact

 

+44 (0)20 7594 3220elaine.holmes

 
 
//

Location

 

661Sir Alexander Fleming BuildingSouth Kensington Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Lamour:2017:infdis/jix466,
author = {Lamour, SD and Alibu, VP and Holmes, E and Sternberg, JM},
doi = {infdis/jix466},
journal = {Journal of Infectious Diseases},
pages = {1273--1280},
title = {Metabolic Profiling of Central Nervous System Disease in Trypanosoma brucei rhodesiense infection},
url = {http://dx.doi.org/10.1093/infdis/jix466},
volume = {216},
year = {2017}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - BACKGROUND: The progression of Human African Trypanosomiasis from the early hemolymphatic stage to the late meningoencephalitic stage is of critical diagnostic importance as it determines the choice of potentially toxic drug regimens. Current diagnostic criteria involving analysis of cerebrospinal fluid (CSF) for parasites and/or pleiocytosis are sensitive, but recent evidence suggests that specificity may be poor.METHODS: We used an untargeted global metabolic profiling approach for the discovery of novel candidate stage diagnostic markers in CSF from Trypanosoma brucei rhodesiense patients using 1H nuclear magnetic resonance (NMR) spectroscopy.RESULTS: Metabolic markers did not resolve early and late stage cases but were associated with neuro-inflammatory responses and the presentation of neurological disturbances. In particular increased 3-hydroxybutyrate and alanine and reduced concentrations of mannose and urea were discriminatory for the presentation of daytime somnolence and gait ataxia.CONCLUSIONS: CSF metabolite concentrations provide markers for neuroinflammatory responses during CNS invasion by trypanosomes and are associated with the presentation of neurological disturbances independently of disease stage determined by current criteria. This suggests that applying a dichotomous stage diagnosis on the basis of CSF pleiocytosis does not accurately reflect the biological changes occurring as parasites invade the CNS and has implications for biomarker discovery strategies.
AU  - Lamour,SD
AU  - Alibu,VP
AU  - Holmes,E
AU  - Sternberg,JM
DO  - infdis/jix466
EP  - 1280
PY  - 2017///
SN  - 0022-1899
SP  - 1273
TI  - Metabolic Profiling of Central Nervous System Disease in Trypanosoma brucei rhodesiense infection
T2  - Journal of Infectious Diseases
UR  - http://dx.doi.org/10.1093/infdis/jix466
UR  - http://hdl.handle.net/10044/1/50549
VL  - 216
ER  -
Download