Imperial College London
Dr Emrys Jones

DrEmrysJones

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Honorary Research Associate
 
 
 
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Contact

 

emrys.jones

 
 
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Location

 

Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Golf:2015:10.1021/ac5046752,
author = {Golf, O and Strittmatter, N and Karancsi, T and Pringle, SD and Speller, AVM and Mroz, A and Kinrosss, JM and Abbassi-Ghadi, N and Jones, EA and Takats, Z},
doi = {10.1021/ac5046752},
journal = {Analytical Chemistry},
pages = {2527--2534},
title = {Rapid evaporative ionization mass spectrometry imaging platform for direct mapping from bulk tissue and bacterial growth media},
url = {http://dx.doi.org/10.1021/ac5046752},
volume = {87},
year = {2015}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Rapid evaporative ionization mass spectrometry (REIMS) technology allows real time intraoperative tissue classification and the characterization and identification of microorganisms. In order to create spectral libraries for training the classification models, reference data need to be acquired in large quantities as classification accuracy generally improves as a function of number of training samples. In this study, we present an automated high-throughput method for collecting REIMS data from heterogeneous organic tissue. The underlying instrumentation consists of a 2D stage with an additional high-precision z-axis actuator that is equipped with an electrosurgical diathermy-based sampling probe. The approach was validated using samples of human liver with metastases and bacterial strains, cultured on solid medium, belonging to the species P. aeruginosa, B. subtilis, and S. aureus. For both sample types, spatially resolved spectral information was obtained that resulted in clearly distinguishable multivariate clustering between the healthy/cancerous liver tissues and between the bacterial species.
AU  - Golf,O
AU  - Strittmatter,N
AU  - Karancsi,T
AU  - Pringle,SD
AU  - Speller,AVM
AU  - Mroz,A
AU  - Kinrosss,JM
AU  - Abbassi-Ghadi,N
AU  - Jones,EA
AU  - Takats,Z
DO  - 10.1021/ac5046752
EP  - 2534
PY  - 2015///
SN  - 0003-2700
SP  - 2527
TI  - Rapid evaporative ionization mass spectrometry imaging platform for direct mapping from bulk tissue and bacterial growth media
T2  - Analytical Chemistry
UR  - http://dx.doi.org/10.1021/ac5046752
UR  - http://hdl.handle.net/10044/1/45378
VL  - 87
ER  -
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