Imperial College London
Dr Emrys Jones

DrEmrysJones

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Honorary Research Associate
 
 
 
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Contact

 

emrys.jones

 
 
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Location

 

Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Yan:2017:10.1021/jacs.6b12165,
author = {Yan, C and Parmeggiani, F and Jones, EA and Claude, E and Hussain, SA and Turner, NJ and Flitsch, SL and Barran, PE},
doi = {10.1021/jacs.6b12165},
journal = {J Am Chem Soc},
pages = {1408--1411},
title = {Real-Time Screening of Biocatalysts in Live Bacterial Colonies.},
url = {http://dx.doi.org/10.1021/jacs.6b12165},
volume = {139},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Screening of bacterial colonies to identify new biocatalytic activities is a widely adopted tool in biotechnology, but is constrained by the requirements for colorimetric or tag-based detection methods. Herein we report a label-free screening platform for biotransformations in live colonies using desorption electrospray ionization coupled with ion mobility mass spectrometry imaging (DiBT-IMMS). The screening method is demonstrated for both ammonia lyases and P450 monooxygenases expressed within live bacterial colonies and is shown to enable multiplexing of enzyme variants and substrate libraries simultaneously.
AU  - Yan,C
AU  - Parmeggiani,F
AU  - Jones,EA
AU  - Claude,E
AU  - Hussain,SA
AU  - Turner,NJ
AU  - Flitsch,SL
AU  - Barran,PE
DO  - 10.1021/jacs.6b12165
EP  - 1411
PY  - 2017///
SP  - 1408
TI  - Real-Time Screening of Biocatalysts in Live Bacterial Colonies.
T2  - J Am Chem Soc
UR  - http://dx.doi.org/10.1021/jacs.6b12165
UR  - https://www.ncbi.nlm.nih.gov/pubmed/28084735
VL  - 139
ER  -
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