Imperial College London
Dr Enrique Martinez-Perez

DrEnriqueMartinez-Perez

Faculty of Medicine, Institute of Clinical Sciences

Reader in Chromosome Biology
 
 
 
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Contact

 

+44 (0)20 3313 4314enrique.martinez-perez Website

 
 
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Location

 

Hammersmith HospitalHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Weick:2014:10.1101/gad.238105.114,
author = {Weick, E-M and Sarkies, P and Silva, N and Chen, RA and Moss, SMM and Cording, AC and Ahringer, J and Martinez-Perez, E and Miska, EA},
doi = {10.1101/gad.238105.114},
journal = {Genes and Development},
pages = {783--796},
title = {PRDE-1 is a nuclear factor essential for the biogenesis of Ruby motif-dependent piRNAs in C-elegans},
url = {http://dx.doi.org/10.1101/gad.238105.114},
volume = {28},
year = {2014}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Piwi-interacting RNAs (piRNA) are small regulatory RNAs with essential roles in maintaining genome integrity in animals and protists. Most Caenorhabditis elegans piRNAs are transcribed from two genomic clusters that likely contain thousands of individual transcription units; however, their biogenesis is not understood. Here we identify and characterize prde-1 (piRNA silencing-defective) as the first essential C. elegans piRNA biogenesis gene. Analysis of prde-1 provides the first direct evidence that piRNA precursors are 28- to 29-nucleotide (nt) RNAs initiating 2 nt upstream of mature piRNAs. PRDE-1 is a nuclear germline-expressed protein that localizes to chromosome IV. PRDE-1 is required specifically for the production of piRNA precursors from genomic loci containing an 8-nt upstream motif, the Ruby motif. The expression of a second class of motif-independent piRNAs is unaffected in prde-1 mutants. We exploited this finding to determine the targets of the motif-independent class of piRNAs. Together, our data provide new insights into both the biogenesis and function of piRNAs in gene regulation.
AU  - Weick,E-M
AU  - Sarkies,P
AU  - Silva,N
AU  - Chen,RA
AU  - Moss,SMM
AU  - Cording,AC
AU  - Ahringer,J
AU  - Martinez-Perez,E
AU  - Miska,EA
DO  - 10.1101/gad.238105.114
EP  - 796
PY  - 2014///
SN  - 0890-9369
SP  - 783
TI  - PRDE-1 is a nuclear factor essential for the biogenesis of Ruby motif-dependent piRNAs in C-elegans
T2  - Genes and Development
UR  - http://dx.doi.org/10.1101/gad.238105.114
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000334354700010&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://genesdev.cshlp.org/content/28/7/783
VL  - 28
ER  -
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