Imperial College London


Faculty of MedicineDepartment of Surgery & Cancer




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BibTex format

author = {Contractor, KB and Kenny, LM and Stebbing, J and Challapalli, A and Al-Nahhas, A and Palmieri, C and Shousha, S and Lewis, JS and Hogben, K and De, Nguyen Q and Coombes, RC and Aboagye, EO},
title = {Biological basis of [11C]choline-positron emission tomography in patients with breast cancer: comparison with [18F]fluorothymidine positron emission tomography},
url = {},
year = {2011}

RIS format (EndNote, RefMan)

AB - OBJECTIVE: The biological significance of [C]choline (CHO) uptake in human tumours is unclear and probably linked to choline kinase-alpha (CHKalpha) expression and cell proliferation. We directly compared CHO with [F]fluorothymidine (FLT), an imaging biomarker of proliferation, by positron emission tomography (PET) in patients with breast cancer to investigate whether cell proliferation is an important determinant of CHO uptake. Furthermore, we evaluated CHKalpha and the Ki67-labelling index (LIKi67) in tumour biopsies. METHODS: Sequential CHO-PET and FLT-PET within the same imaging session were performed in 21 patients with oestrogen receptor (ER)-positive breast cancer (28 lesions). Average and maximum CHO standardized uptake values (SUV) at 60 min: SUV60,av, and SUV60,max, and the rate constant of net irreversible uptake, Ki, were compared with FLT uptake at 60 min: SUV60,av and SUV60,max. Biopsies were stained for CHKalpha and LIKi67 in eight cases. RESULTS: Tumours were equally visible on CHO-PET and FLT-PET imaging. Tumour CHO-PET strongly correlated with FLT imaging variables (Pearson's r=0.83; P<0.0001 for CHO-SUV60,max vs. FLT-SUV60,max). A statistically significant association was found between CHO-PET variables and categorical scores of cytoplasmic CHKalpha intensity and between FLT-PET and LIKi67 (P<0.05, one-way analysis of variance). CONCLUSION: Choline metabolism and proliferation as assessed by PET were correlated in ER-positive breast cancer, indicating that high CHO uptake is a measure of cellular proliferation in this setting. CHO uptake was also found to be related to cytoplasmic CHKalpha expression.
AU - Contractor,KB
AU - Kenny,LM
AU - Stebbing,J
AU - Challapalli,A
AU - Al-Nahhas,A
AU - Palmieri,C
AU - Shousha,S
AU - Lewis,JS
AU - Hogben,K
AU - De,Nguyen Q
AU - Coombes,RC
AU - Aboagye,EO
PY - 2011///
SN - 1473-5628
TI - Biological basis of [11C]choline-positron emission tomography in patients with breast cancer: comparison with [18F]fluorothymidine positron emission tomography
UR -
ER -