Imperial College London

ProfessorEricAboagye

Faculty of MedicineDepartment of Surgery & Cancer

Professor
 
 
 
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Contact

 

+44 (0)20 3313 3759eric.aboagye

 
 
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Assistant

 

Mrs Maureen Francis +44 (0)20 7594 2793

 
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Location

 

GN1Commonwealth BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Dart:2013:10.1371/journal.pone.0071694,
author = {Dart, DA and Waxman, J and Aboagye, EO and Bevan, CL},
doi = {10.1371/journal.pone.0071694},
journal = {PLOS One},
title = {Visualising Androgen Receptor Activity in Male and Female Mice},
url = {http://dx.doi.org/10.1371/journal.pone.0071694},
volume = {8},
year = {2013}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Androgens, required for normal development and fertility of males and females, have vital roles in the reproductivetract, brain, cardiovascular system, smooth muscle and bone. Androgens function via the androgen receptor (AR), aligand-dependent transcription factor. To assay and localise AR activity in vivo we generated the transgenic “ARELuc”mouse, expressing a luciferase reporter gene under the control of activated endogenous AR. In vivo imaging ofandrogen-mediated luciferase activity revealed several strongly expressing tissues in the male mouse as expectedand also in certain female tissues. In males the testes, prostate, seminal vesicles and bone marrow all showed highAR activity. In females, strong activity was seen in the ovaries, uterus, omentum tissue and mammary glands. In bothsexes AR expression and activity was also found in salivary glands, the eye (and associated glands), adipose tissue,spleen and, notably, regions of the brain. Luciferase protein expression was found in the same cell layers asandrogen receptor expression. Additionally, mouse AR expression and activity correlated well with AR expression inhuman tissues. The anti-androgen bicalutamide reduced luciferase signal in all tissues. Our model demonstrates thatandrogens can act in these tissues directly via AR, rather than exclusively via androgen aromatisation to estrogensand activation of the estrogen receptor. Additionally, it visually demonstrates the fundamental importance of ARsignalling outside the normal role in the reproductive organs. This model represents an important tool forphysiological and developmental analysis of androgen signalling, and for characterization of known and novelandrogenic or antiandrogenic compounds.
AU - Dart,DA
AU - Waxman,J
AU - Aboagye,EO
AU - Bevan,CL
DO - 10.1371/journal.pone.0071694
PY - 2013///
SN - 1932-6203
TI - Visualising Androgen Receptor Activity in Male and Female Mice
T2 - PLOS One
UR - http://dx.doi.org/10.1371/journal.pone.0071694
UR - http://hdl.handle.net/10044/1/29639
VL - 8
ER -