Imperial College London
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DrFrancescaCeroni

Faculty of Engineering, Department of Chemical Engineering

Senior Lecturer
 
 
 
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Contact

 

f.ceroni

 
 
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Location

 

510AACE ExtensionSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Enrico:2018:10.3389/fbioe.2018.00077,
author = {Enrico, Bena C and Grob, A and Isalan, M and Bosia, C and Ceroni, F},
doi = {10.3389/fbioe.2018.00077},
journal = {Frontiers in Bioengineering and Biotechnology},
title = {Commentary: Synthetic Addiction Extends the Productive Life Time of Engineered Escherichia coli Populations},
url = {http://dx.doi.org/10.3389/fbioe.2018.00077},
volume = {6},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - A commentary on Synthetic addiction extends the productive life time of engineered Escherichia coli populations by Rugbjerg, P., Sarup-Lytzen, K., Nagy, M., and Sommer, M. O. A. (2018). Proc. Natl. Acad. Sci. U.S.A. 115, 2347–2352. doi: 10.1073/pnas.1718622115Bioproduction is the process of producing added-value chemicals on large-scale using cells as biological factories. Cellular burden represents a significant problem in the scaling of fermentation processes from proof-of-concept to long-term cultures, as the load of heterologous gene expression and depletion of the cell intracellular resources cause unpredictable cellular physiological changes that can lead to decreased growth and lower production yields (Borkowski et al., 2016; Liu et al., 2018). One possible cause of the observed decreased bioproduct recovery in many bioprocessing applications is the accumulation of mutations in the employed genetic program. These mutations often lead to loss of production and rise of non-producing populations that grow better and easily overtake the growth of producing cells (Rugbjerg et al., 2018b).In a recent paper in PNAS, Rugbjerg et al. (2018b) developed a strategy to limit the enrichment of non-producing cell populations in bioproduction-employed cell cultures by placing the genes for key growth intermediates under the control of a promoter responsive to the bioproduct being made. This strategy known as product addiction was tested in E. coli engineered to produce mevalonic acid in long-term cultivations (Figure 1).
AU  - Enrico,Bena C
AU  - Grob,A
AU  - Isalan,M
AU  - Bosia,C
AU  - Ceroni,F
DO  - 10.3389/fbioe.2018.00077
PY  - 2018///
SN  - 2296-4185
TI  - Commentary: Synthetic Addiction Extends the Productive Life Time of Engineered Escherichia coli Populations
T2  - Frontiers in Bioengineering and Biotechnology
UR  - http://dx.doi.org/10.3389/fbioe.2018.00077
UR  - http://hdl.handle.net/10044/1/60319
VL  - 6
ER  -
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